Cytoprotective and anti-inflammatory effects of PAL31 overexpression in glial cells

Journal of Biomedical Science

Henrich Cheng²  Wen-Cheng Huang²  May-Jywan Tsai³  Dann-Ying Liou³  Fan-Wei Tseng¹ 

[1] Neural Regeneration Laboratory, Neurological Institute, Taipei Veterans General Hospital, No 322, Shih-Pai Road, Sec. 2, Taipei 112, Taiwan;Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan;Center for Neural Regeneration, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan

关键词: Microglia;    C6 glioma;    Astroglia;    H₂O₂ toxicity;    Inflammation;    Proliferation related acidic leucine-rich protein;    Spinal cord injury;   

Others  :  1137508
DOI  :  10.1186/1423-0127-21-60

 received in 2014-03-13, accepted in 2014-06-01,  发布年份 2014

【 摘 要 】

Background

Acute spinal cord injury (SCI) leads to a series of reactive changes and causes severe neurological deficits. A pronounced inflammation contributes to secondary pathology after SCI. Astroglia respond to SCI by proliferating, migrating, and altering phenotype. The impact of reactive gliosis on the pathogenesis of SCI is not fully understood. Our previous study has identified an inflammatory modulating protein, proliferation related acidic leucine-rich protein (PAL31) which is upregulated in the microglia/macrophage of injured cords. Because PAL31 participates in cell cycle progression and reactive astroglia often appears in the injured cord, we aim to examine whether PAL31 is involved in glial modulation after injury.

Results

Enhanced PAL31 expression was shown not only in microglia/macrophages but also in spinal astroglia after SCI. Cell culture study reveal that overexpression of PAL31 in mixed glial cells or in C6 astroglia significantly reduced LPS/IFNγ stimulation. Further, enhanced PAL31 expression in C6 astroglia protected cells from H₂O₂ toxicity; however, this did not affect its proliferative activity. The inhibiting effect of PAL31 on LPS/IFNγ stimulation was observed in glia or C6 after co-culture with neuronal cells. The results demonstrated that the overexpressed PAL31 in glial cells protected neuronal damages through inhibiting NF-kB signaling and iNOS.

Conclusions

Our data suggest that PAL31upregulation might be beneficial after spinal cord injury. Reactive gliosis might become a good target for future therapeutic interventions.

【 授权许可】

   
2014 Tseng et al.; licensee BioMed Central Ltd.

【 图 表 】

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