Differential expression of major histocompatibility complex class I in developmental glioneuronal lesions

Journal of Neuroinflammation

Eleonora Aronica¹  Peter C van Rijen²  Wim GM Spliet²  Jasper J Anink³  Anand M Iyer³  Avanita S Prabowo³ 

[1] Swammerdam Institute for Life Sciences, Center for Neuroscience, University of Amsterdam, Amsterdam, The Netherlands;Departments of Pathology and Neurosurgery /Rudolf Magnus Institute for Neuroscience, University Medical Center Utrecht, Utrecht, The Netherlands;Department of (Neuro)Pathology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, Amsterdam, 1105 AZ, The Netherlands

关键词: Major histocompatibility complex (MHC) class I;    Microglia;    Neurons;    Cortical tubers;    Ganglioglioma;    Focal cortical dysplasia;   

Others  :  1160059
DOI  :  10.1186/1742-2094-10-12

 received in 2012-11-14, accepted in 2013-01-10,  发布年份 2013

【 摘 要 】

Purpose

The expression of the major histocompatibility complex class I (MHC-I) in the brain has received considerable interest not only because of its fundamental role in the immune system, but also for its non-immune functions in the context of activity-dependent brain development and plasticity.

Methods

In the present study we evaluated the expression and cellular pattern of MHC-I in focal glioneuronal lesions associated with intractable epilepsy. MHC-I expression was studied in epilepsy surgery cases with focal cortical dysplasia (FCD I, n = 6; FCD IIa, n = 6 and FCD IIb, n = 15), tuberous sclerosis complex (TSC, cortical tubers; n = 6) or ganglioglioma (GG; n = 15) using immunocytochemistry. Evaluation of T lymphocytes with granzyme-B⁺ granules and albumin immunoreactivity was also performed.

Results

All lesions were characterized by MHC-I expression in blood vessels. Expression in both endothelial and microglial cells as well as in neurons (dysmorphic/dysplastic neurons) was observed in FCD II, TSC and GG cases. We observed perivascular and parenchymal T lymphocytes (CD8⁺, T-cytotoxic) with granzyme-B⁺ granules in FCD IIb and TSC specimens. Albumin extravasation, with uptake in astrocytes, was observed in FCD IIb and GG cases.

Conclusions

Our findings indicate a prominent upregulation of MHC-I as part of the immune response occurring in epileptogenic glioneuronal lesions. In particular, the induction of MHC-I in neuronal cells appears to be a feature of type II FCD, TSC and GG and may represent an important accompanying event of the immune response, associated with blood–brain barrier dysfunction, in these developmental lesions.

【 授权许可】

   
2013 Prabowo et al.; licensee BioMed Central Ltd.

【 图 表 】

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