DNA and RNA analyses in detection of genetic predisposition to cancer

Hereditary Cancer in Clinical Practice

Rodney J Scott¹  Anna Jakubowska²  Bartłomiej Masojć²  Joanna Trubicka³  Pablo Serrano-Fernández²  Dagmara Dymerska²  Grzegorz Kurzawski² 

[1] The Division of Genetics, Hunter Area Pathology Service, John Hunter Hospital, Newcastle, Australia;International Hereditary Cancer Center, Department of Genetics and Pathology, Pomeranian Medical University, Szczecin, Poland;Department of Medical Genetics, the Children’s Memorial Health Institute, Warsaw, Poland

关键词: Diagnoses;    Techniques;    Hereditary cancer;    Constitutional changes;   

Others  :  806219
DOI  :  10.1186/1897-4287-10-17

 received in 2012-10-25, accepted in 2012-11-24,  发布年份 2012

【 摘 要 】

During the past decade many new molecular methods for DNA and RNA analysis have emerged. The most popular thus far have been SSCP, HET, CMC, DGGE, RFLP or ASA, which have now been replaced by methods that are more cost effective and less time consuming. Real-time amplification techniques and particularly those with the capacity of multiplexing have become commonly used in laboratory practice. Novel screening methods enable the very rapid examination of large patients series. Use of liquid handling robotics applied to the isolation of DNA or RNA, the normalisation of sample concentration, and standardization of target amplification by PCR have also contributed to a reduced risk of sample contamination and have resulted in laboratory analysis being easier and faster.

The aim of this study is the introduction of a few modern techniques, most commonly used in detection of genetic predisposition to cancer.

【 授权许可】

   
2012 Kurzawski et al.; licensee BioMed Central Ltd.

【 图 表 】

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