Functional Impact of RNA editing and ADARs on regulation of gene expression: perspectives from deep sequencing studies

Cell & Bioscience

Bertrand Chin-Ming Tan³  Kai-Ping Chang⁴  Scott C Schuyler¹  Yi-Tung Chen²  Chung-Pei Ma²  Hsuan Liu³ 

[1] Department of Biomedical Sciences, College of Medicine, Tao-Yuan, Taiwan;Graduate Institute of Biomedical Sciences, Tao-Yuan, Taiwan;Molecular Medicine Research Center, Chang Gung University, Tao-Yuan, Taiwan;Department of Otolaryngology, Chang Gung Memorial Hospital at Lin-Kuo, Tao-Yuan, Taiwan

关键词: Transcriptional regulation;    Small RNA-seq;    RNA editing;    RNA-seq;    Next generation sequencing (NGS);    MicroRNA;    Gene regulation;    Alternative splicing;    ADAR;   

Others  :  1149438
DOI  :  10.1186/2045-3701-4-44

 received in 2014-05-13, accepted in 2014-07-14,  发布年份 2014

【 摘 要 】

Cells regulate gene expression at multiple levels leading to a balance between robustness and complexity within their proteome. One core molecular step contributing to this important balance during metazoan gene expression is RNA editing, such as the co-transcriptional recoding of RNA transcripts catalyzed by the adenosine deaminse acting on RNA (ADAR) family of enzymes. Understanding of the adenosine-to-inosine RNA editing process has been broadened considerably by the next generation sequencing (NGS) technology, which allows for in-depth demarcation of an RNA editome at nucleotide resolution. However, critical issues remain unresolved with regard to how RNA editing cooperates with other transcript-associated events to underpin regulated gene expression. Here we review the growing body of evidence, provided by recent NGS-based studies, that links RNA editing to other mechanisms of post-transcriptional RNA processing and gene expression regulation including alternative splicing, transcript stability and localization, and the biogenesis and function of microRNAs (miRNAs). We also discuss the possibility that systematic integration of NGS data may be employed to establish the rules of an “RNA editing code”, which may give us new insights into the functional consequences of RNA editing.

【 授权许可】

   
2014 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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