Bison and bovine rectoanal junctions exhibit similar cellular architecture and Escherichia coli O157 adherence patterns

BMC Veterinary Research

Judith A Stasko¹  Indira T Kudva² 

[1] Microscopy Services Laboratory, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, IA 50010, USA;Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, IA 50010, USA

关键词: Adherence;    Markers;    Epithelia;    Tissue;    Bison;    Bovine;    O157:H7;   

Others  :  1119372
DOI  :  10.1186/1746-6148-9-266

 received in 2013-10-21, accepted in 2013-12-18,  发布年份 2013

【 摘 要 】

Background

Escherichia coli O157 (E. coli O157) has been isolated from bison retail meat, a fact that is important given that bison meat has been implicated in an E. coli O157-multistate outbreak. In addition, E. coli O157 has also been isolated from bison feces at slaughter and on farms. Cattle are well documented as E. coli O157 reservoirs, and the primary site of E. coli O157 persistence in such reservoirs is the rectoanal junction (RAJ), located at the distal end of the bovine gastrointestinal tract. Since bison and cattle share many genetic similarities manifested as common lineage, susceptibility to infection and the nature of immune responses to infectious agents, we decided to evaluate whether the RAJ of these animals were comparable both in terms of cellular architecture and as sites for adherence of E. coli O157. Specifically, we compared the histo-morphologies of the RAJ and evaluated the E. coli O157 adherence characteristics to the RAJ squamous epithelial (RSE) cells, from these two species.

Results

We found that the RAJ of both bison and cattle demonstrated similar distribution of epithelial cell markers villin, vimentin, cytokeratin, E-cadherin and N-cadherin. Interestingly, N-cadherin predominated in the stratified squamous epithelium reflecting its proliferative nature. E. coli O157 strains 86–24 Sm^R and EDL 933 adhered to RSE cells from both animals with similar diffuse and aggregative patterns, respectively.

Conclusion

Our observations further support the fact that bison are likely ‘wildlife’ reservoirs for E. coli O157, harboring these bacteria in their gastrointestinal tract. Our results also extend the utility of the RSE-cell assay, previously developed to elucidate E. coli O157-cattle RAJ interactions, to studies in bison, which are warranted to determine whether these observations in vitro correlate with those occurring in vivo at the RAJ within the bison gastrointestinal tract.

【 授权许可】

   
2013 Kudva and Stasko; licensee BioMed Central Ltd.

【 图 表 】

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