Effect of the ionic product of bioglass 60s on osteoblastic activity in canines

BMC Veterinary Research

Cleuza M F Rezende²  Alfredo M. Góes¹  Humberto P. Oliveira²  Natália M. Ocarino²  Marivalda M. Pereira³  Isabel R. Rosado²  Rogéria Serakides²  Endrigo G L Alves² 

[1] Department of Biochemistry and Immunology of the Institute of Biological Sciences at UFMG, Belo Horizonte, Brazil;Center for Stem Cells and Animal Cell Therapy (Núcleo de Células Tronco e Terapia Celular Animal - NCT-TCA), Department of Medicine and Surgery, Veterinary School of the Federal University of Minas Gerais (Universidade Federal de Minas Gerais - UFMG), Belo Horizonte, Brazil;Laboratory of Biomaterials of the Department of Metallurgic and Materials Engineering at UFMG, Belo Horizonte, Brazil

关键词: Osteoblasts;    Bone substitute;    Biomaterials;    Orthopaedics;   

Others  :  1228786
DOI  :  10.1186/s12917-015-0558-7

 received in 2014-11-25, accepted in 2015-09-21,  发布年份 2015

【 摘 要 】

Background

The objective of the present study was to evaluate the effect of the ionic product (IP ) of BG60S on osteoblastic activity. The following media groups were created: DMEM , which is formed by osteoblasts in basal medium; IP DMEM , which is formed by osteoblasts in IP with basal medium; OST , which is formed by osteoblasts in osteogenic medium; and IP OST , which is formed by osteoblasts in IP with osteogenic medium. The osteoblasts were cultivated in an incubator at 37 °C and 5 % CO ₂for 7, 14 and 21 days. After each period, the alkaline phosphatase (AP) activity, mineralised area per field and expression of osterix (OSX), bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC) were evaluated by reverse transcription (RT)-PCR.

Results

The IP significantly increased the AP activity in the IP DMEM group at 7 and 14 days and reduced the AP activity in the IP OST group at 14 and 21 days relative to their respective controls (DMEM and OST). The groups that received the IP displayed a significant increase in the percentage of mineralised area per field and more advance maturation of the extracellular matrix relative to those that did not receive IP. The IP significantly increased the expression of OSX, BSP and ON in osteoblast cultures maintained in IP DMEM compared with the control (DMEM) for the majority of studied periods. In osteogenic medium, IP also significantly increased OSX, BSP, ON and OC expression compared with the control (OST) for the majority of studied periods.

Conclusions

The IP of BG60S alters the gene expression of canine osteoblasts, favouring the synthesis and mineralisation of the extracellular matrix.

【 授权许可】

   
2015 Alves et al.

【 图 表 】

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