Is hepatic lipid metabolism of beef cattle influenced by breed and dietary silage level?

BMC Veterinary Research

José António Mestre Prates¹  Carlos Mendes Godinho Andrade Fontes¹  Rui Manuel Amaro Pinto²  Eva Alves Rolo¹  Virgínia Maria Rico Pires¹  Rui José Branquinho Bessa¹  Ana Sofia Henriques da Costa¹ 

[1] CIISA, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, Pólo Universitário do Alto da Ajuda, 1300-477 Lisboa, Portugal;iMed.UL, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, Portugal

关键词: Gene expression;    Fatty acids;    Beef cattle;    Liver;   

Others  :  1119305
DOI  :  10.1186/1746-6148-10-65

 received in 2013-05-08, accepted in 2014-03-05,  发布年份 2014

【 摘 要 】

Background

In ruminants, unsaturated dietary fatty acids are biohydrogenated in the rumen and are further metabolised in various tissues, including liver, which has an important role in lipid and lipoprotein metabolism. Therefore, manipulation of muscle fatty acid composition should take into account liver metabolism. In the present study, the influence of breed and diet on liver lipid composition and gene expression was investigated in order to clarify the role of this organ in the lipid metabolism of ruminants. Forty purebred young bulls from two phylogenetically distant autochthonous cattle breeds, Alentejana and Barrosã, were assigned to two different diets (low vs. high silage) and slaughtered at 18 months of age. Liver fatty acid composition, mRNA levels of enzymes and transcription factors involved in lipid metabolism, as well as the plasma lipid profile, were assessed.

Results

In spite of similar plasma non-esterified fatty acids levels, liver triacylglycerols content was higher in Barrosã than in Alentejana bulls. Moreover, the fatty acid composition of liver was clearly distinct from the remaining tissues involved in fatty acid metabolism of ruminants, as shown by Principal Components Analysis. The hepatic tissue is particularly rich in α-linolenic acid and their products of desaturation and elongation. Results indicate that DGAT1, ELOVL2, FADS1 and FADS2 genes influence the fatty acid composition of the liver the most. Moreover, genes such as DGAT1 and ELOVL2 appear to be more sensitive to genetic background than to dietary manipulation, whereas genes encoding for desaturases, such as FADS1, appear to be modulated by dietary silage level.

Conclusions

Our results indicate that liver plays an important role in the biosynthesis of n-3 LC-PUFA. It is also suggested that dietary silage level influences the hepatic fatty acid metabolism in a breed-dependent manner, through changes in the expression of genes encoding for enzymes associated with the desaturation and elongation pathway. The importance of devising custom-made feeding strategies taking into account the genetic background is, therefore, stressed by the results from this experiment.

【 授权许可】

   
2014 da Costa et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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