Increased fluidity and oxidation of malarial lipoproteins: relation with severity and induction of endothelial expression of adhesion molecules

Lipids in Health and Disease

Rachanee Udomsangpetch⁴  Sornchai Looareesuwan²  Gary Brittenham¹  Wattana Leowattana²  Srivicha Krudsood²  Paveena Yamanont³  Nathawut Sibmooh³ 

[1] Department of Medicine and Pediatrics, Columbia University, College of Physicians and Surgeons, New York, USA;Department of Clinical Tropical Medicine, Hospital for Tropical Diseases, Faculty of Tropical Medicine, Mahidol University, Thailand;Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand;Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand

关键词: adhesion molecules;    endothelial cell;    oxidized LDL;    lipoprotein;    lipid profile;    oxidative stress;    malaria;   

Others  :  1213291
DOI  :  10.1186/1476-511X-3-15

 received in 2004-05-20, accepted in 2004-06-25,  发布年份 2004

【 摘 要 】

Introduction

Oxidative stress has been demonstrated in malaria. The potential oxidative modification of lipoproteins derived from malaria patients was studied. These oxidized lipids may have role in pathogenesis of malaria.

Method

The plasma lipid profile and existence of oxidized forms of very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoprotein (HDL) were investigated in malaria (17 mild and 24 severe patients) and 37 control subjects. Thiobarbituric acid reactive substances (TBARs), conjugated dienes, tryptophan fluorescence and fluidity of lipoproteins were determined as markers of oxidation. The biological effect of malarial lipoproteins was assessed by the expression of adhesion molecules on endothelial cells.

Results

Malarial lipoproteins had decreased cholesterol (except in VLDL) and phospholipid. The triglyceride levels were unchanged. The cholesterol/phospholipid ratio of LDL was decreased in malaria, but increased in VLDL and HDL. TBARs and conjugate dienes were increased in malarial lipoproteins, while the tryptophan fluorescence was decreased. The fluidity of lipoproteins was increased in malaria. These indicated the presence of oxidized lipoproteins in malaria by which the degree of oxidation was correlated with severity. Of three lipoproteins from malarial patients, LDL displayed the most pronounced oxidative modification. In addition, oxidized LDL from malaria patients increased endothelial expression of adhesion molecules.

Conclusion

In malaria, the lipoproteins are oxidatively modified, and the degree of oxidation is related with severity. Oxidized LDL from malarial patients increases the endothelial expression of adhesion molecules. These suggest the role of oxidized lipoproteins, especially LDL, on the pathogenesis of disease.

【 授权许可】

   
2004 Sibmooh et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

【 图 表 】

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