Effect of pre-milking teat preparation procedures on the microbial count on teats prior to cluster application

Irish Veterinary Journal

F Galli¹  E O'Callaghan¹  J Flynn¹  B O'Brien¹  D Gleeson¹ 

[1] Teagasc, Moorepark, Dairy Production Research Centre, Fermoy, Co. Cork

关键词: teat preparation;    swabs;    microbial counts;    cows;   

Others  :  1149798
DOI  :  10.1186/2046-0481-62-7-461

【 摘 要 】

A study was carried out to investigate the effect of six pre-milking teat preparation procedures on lowering the staphylococal, streptococcal and coliform microbial count on teat skin prior to cluster application. The teat preparations included 'Iodine', 'Chlorhexidine' teat foam, 'Washing and drying' with paper, 'No preparation', 'Chlorine' teat foam, and disinfectant 'Wipes'. Teat preparations were applied for five days to 10 cows for each treatment during two herd management periods (indoors and outdoors). Teats were swabbed on day four and five before teat preparation and repeated after teat preparation. The swabs were plated on three selective agars: Baird Parker (Staphylococcus spp.), Edwards (Streptococcus spp.), and MacConkey (coliform). Following incubation, microbial counts for each pathogen type were manually counted and assigned to one of six categories depending on the microbial counts measured. The results were analysed by logistic regression using SAS [28]. The main analysis was conducted on binary improvement scores for the swabbing outcomes. There were no differences for staphylococcal, streptococcal and coliform bacterial counts between treatments, measured 'before' teat preparation. Treatments containing 'Chlorhexidine' teat foam (OR = 4.46) and 'Wipes' (OR = 4.46) resulted in a significant reduction (P < 0.01) in the staphylococcal count on teats compared to 'Washing and drying' or 'No preparation'. 'Chlorine' teat foam (OR = 3.45) and 'Wipes' (3.45) had the highest probability (P < 0.01) of reducing streptococcal counts compared to 'Washing and drying' or 'No preparation'. There was no statistical difference between any of the disinfectant treatments applied in reducing coliforms. Thus, the use of some disinfectant products for pre-milking teat preparation can have beneficial effects on reducing the levels of staphylococcal and streptococcal pathogens on teat skin.

   

【 参考文献 】

• [1]Barkema HW, Schukken YH, Lam TJGM, et al.: Management practices associated with the incidence rate of clinical mastitis. Journal of Dairy Science 1999, 82:1643-1654.
• [2]Berry DP, Amer PR: Proceedings of the Irish Grassland and Annual Production Association. Tullamore 2006, 15-16. March, 32, 80
• [3]Foret CJ, Corbellini C, Young S, et al.: Efficacy of two iodine teat dips based on reduction of naturally occurring new intramammary infections. Journal of Dairy Science 2005, 88:426-432.
• [4]Galton DM, Adkinson RW, Thomas CV, et al.: Effects of pre-milking udder preparation on environmental bacterial contamination of milk. Journal of Dairy Science 1982, 65:1540-1543.
• [5]Galton DM, Petersson LG, Merrill WG, et al.: Effects of pre-milking udder preparation on bacterial population, sediment and iodine residues in milk. Journal of Dairy Science 1984, 67:2580-2589.
• [6]Galton DM, Petersson LG, Merrill WG: Effects of pre-milking udder preparation practices on bacterial counts in milk and teats. Journal of Dairy Science 1986, 69:260-266.
• [7]Gibson H, Sinclair LA, Brizuela CM, et al.: Effectiveness of selected pre-milking teat-cleaning regimes in reducing teat microbial load on commercial dairy farms. Letters in Applied Microbiology 2008, 46:295-300.
• [8]Gleeson DE, Meaney WJ, O'Callaghan EJ: Effect of post-milking teat disinfectant on the relationship between teat hyperkeratosis, somatic cell count and the incidence of mastitis. Proceedings of physiological and technical aspects of machine milking, Nitra, Slovak Republic, 26-28 April, 10 2004, 225-227.
• [9]Grindal RJ, Bramley AJ: Effect of udder preparation on transmission of Staphylococcus aureus while milking with a multi-valved cluster. Journal of Dairy Research 1989, 56:683-690.
• [10]Heinze G, Schemper M: A solution to the problem of separation in logistic regression. Statistics in Medicine 2002, 21:2409-2419.
• [11]Hutchison ML, Thomas DJI, Moore A, et al.: An evaluation of raw microorganisms as markers of on-farm hygiene practices related to milking. Journal of Food Protection 2005, 68:764-772.
• [12]Ingawa KH, Adkinson RW, Gough RH: Evaluation of a gel teat cleaning and sanitising compound for premilking hygene. Journal of Dairy Science 1992, 75:1224-1232.
• [13]Kagkli DM, Vancanneyt M, Vandamme P, et al.: Contamination of milk by enterococci and coliforms from bovine faeces. Journal of Applied Microbiology 2007, 103:1393-1405.
• [14]Kelly PJ, O'Sullivan K, Berry DP, et al.: Farm management factors associated with bulk tank total bacterial count in Irish dairy herds during 2006/07. Irish Veterinary Journal 2009, 62(1):36-42.
• [15]Magnusson M, Christiansson A, Svensson B, et al.: Effect of different pre-milking manual teat-cleaning methods on bacterial spore milk. Journal of Dairy Science 2006, 89:3866-3875.
• [16]Matthews KR, Harmon RJ, Langlois BE: Prevalence of Staphylococcus species during the periparturient period in primiparous and multiparous cows. Journal of Dairy Science 1992, 75:1835-1839.
• [17]McKinnon CH, Rowlands GJ, Bramley AJ: The effect of udder preparation before milking and contamination from the milking plant on bacterial numbers in bulk milk of eight dairy herds. Journal of Dairy Research 1990, 57:307-318.
• [18]Miller RH, Paape MJ, Fulton LA, et al.: The relationship of milk somatic cell count to milk yields for Holstein heifers after first calving. Journal of Dairy Science 1993, 76:728-733.
• [19]Murphy PM, Freyne T, Gleeson D, et al.: Impact of pre-milking teat preparation practices on milk quality. Proceedings of Physiological and technical aspects of machine milking, Nitra, Slovak Republic, 26-28 April, ICAR Technical Series 10 2004, 279-280.
• [20]Myllys V, Rautala H: Characterisation of clinical mastitis in primiparous heifers. Journal of Dairy Science 1995, 78:538-545.
• [21]Newbould FHS: Factors contributing to new infections. Proceedings Annual Meeting National Mastitis Council. Washington DC 1970, 3.
• [22]O'Shea J: Machine milking factors affecting mastitis. IDF Bulletin 1987, 215:5-32.
• [23]Oliver SP, Lewis MJ, Ingle TL, et al.: Pre-milking teat disinfection for the prevention of environmental pathogen intramammary infections. Journal of Food Protection 1993, 56:852-855.
• [24]Oliver SP, Gillespie BE, Lewis MJ, et al.: Efficacy of a new pre-milking teat disinfectant containing a phenolic combination for the prevention of mastitis. Journal of Dairy Science 2001, 84:1545-1549.
• [25]O'Brien B: Practical steps to improve milk quality. Milk Quality Handbook. Teagasc 2008, 104.
• [26]Pankey JW, Wildman EE, Drechsler PA, et al.: Field trial evaluation of premilking teat disinfection. Journal of Dairy Science 1987, 70:867-872.
• [27]Roberson JR, Fox LK, Hancock DD, et al.: Ecology of Staphylococcus aureus isolated from various sites on dairy farms. Journal of Dairy Science 1994, 77:3354-3364.
• [28]SAS: SAS/STAT® User's Guide. In Cary. NC: SAS Institute Inc; 2004.
• [29]Vissers MMM, Driehuis F, Te Giffel, et al.: Short communication: quantification of the transmission of microorganism to milk via dirt attached to the exterior of the teats. Journal of Dairy Science 2007, 90:3579-3582.
• [30]Watts JL, Nickerson SC, Boddie RL, et al.: Effects of a 1.94% sulfonic acid teat dip and 1% iodophore teat dip on teat canal infections in lactation dairy cows. Journal of Dairy Science 1991, 74:1115-1123.
• [31]Wilson DJ, Das HH, Gonzalez RN, et al.: Association between management practices, dairy herd characteristics, and somatic cell count of bulk tank milk. Journal of American Veterinary and Medical Association 1997, 210:1499-1502.