Experimental manipulation of radiographic density in mouse mammary gland

Breast Cancer Research

Rama Khokha¹  Norman F Boyd¹  Tak W Mak¹  Martin J Yaffe²  Stephanie A Backman¹  Michelle MacPherson²  Marco A DiGrappa¹  Geoffrey A Wood¹  Mehrdad Hariri¹ 

[1] Department of Medical Biophysics, Ontario Cancer Institute/University Health Network, Toronto, Ontario, Canada;Imaging Research, Sunnybrook and Women's Health Sciences Centre, Toronto, Ontario, Canada

关键词: radiography;    mouse model;    mammography;    mammographic density;    breast cancer;   

Others  :  1118727
DOI  :  10.1186/bcr901

 received in 2004-02-04, accepted in 2004-06-01,  发布年份 2004

【 摘 要 】

Introduction

Extensive mammographic density in women is associated with increased risk for breast cancer. Mouse models provide a powerful approach to the study of human diseases, but there is currently no model that is suited to the study of mammographic density.

Methods

We performed individual manipulations of the stromal, epithelial and matrix components of the mouse mammary gland and examined the alterations using in vivo and ex vivo radiology, whole mount staining and histology.

Results

Areas of density were generated that resembled densities in mammographic images of the human breast, and the nature of the imposed changes was confirmed at the cellular level. Furthermore, two genetic models, one deficient in epithelial structure (Pten conditional tissue specific knockout) and one with hyperplastic epithelium and mammary tumors (MMTV-PyMT), were used to examine radiographic density.

Conclusion

Our data show the feasibility of altering and imaging mouse mammary gland radiographic density by experimental and genetic means, providing the first step toward modelling the biological processes that are responsible for mammographic density in the mouse.

【 授权许可】

   
2004 Hariri et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

【 图 表 】

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