The impact of diurnal variation on induced sputum cell counts in healthy adults

Clinical and Translational Allergy

Richard Leigh¹  Stephanie The¹  Lisa E Wong²  Warren J Davidson¹ 

[1] Department of Medicine, University of Calgary, 7007-14th Street SW, Calgary, Alberta, T2V 1P9, Canada;Faculty of Kinesiology, University of Calgary, Calgary, Alberta, Canada

关键词: Diurnal variation;    Inflammation;    Induced sputum;   

Others  :  794387
DOI  :  10.1186/2045-7022-3-8

 received in 2013-01-11, accepted in 2013-03-16,  发布年份 2013

【 摘 要 】

Background

Induced sputum cell counts are a non-invasive, reliable method for evaluating the presence, type, and degree of inflammation in the airways of the lungs. Current reference values for induced sputum cell counts in healthy adults do not account for the effects of circadian rhythm, including diurnal variation. The objective of this study was to describe the diurnal variation in induced sputum cell counts, compared between early morning and late afternoon, in healthy adult individuals.

Methods

100 healthy adult subjects with no history of lung disease and normal bronchial reactivity proceeded with induced sputum testing at 7 am and 4 pm on different days. The order of testing was randomized. The cytotechnologist preparing and performing the cell counts was blinded to the sample collection time and subject characteristics.

Results

65 subjects were included in the final analyses. There was no significant change in the total and differential sputum cell counts between the 7 am and 4 pm collections. There was good inter-observer agreement with respect to differential sputum cell count interpretation.

Conclusions

This is the largest study to assess the variation in induced sputum cell counts in healthy adult subjects at different times of the day. We found no significant change in total and differential sputum cell counts between the 7 am and 4 pm collection time points. This is in contrast to studies in asthmatics that demonstrated a circadian variation in sputum cell counts and other markers of inflammation, suggesting that fluctuations in airway inflammatory cells during the day are a disease-specific effect.

【 授权许可】

   
2013 Davidson et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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