Circulation of the low pathogenic avian influenza subtype H5N2 virus in ducks at a live bird market in Ibadan, Nigeria

Infectious Diseases of Poverty

David Olaleye¹  Georgina Odaibo¹  Clement Meseko¹  Temitope Coker¹ 

[1] Virology Department, College of Medicine, University of Ibadan, Ibadan, Nigeria

关键词: Nigeria;    Circulation;    Live bird market;    Domestic ducks;    LPAI H5N2;   

Others  :  1136013
DOI  :  10.1186/2049-9957-3-38

 received in 2014-04-09, accepted in 2014-10-08,  发布年份 2014

【 摘 要 】

Background

To monitor carrier hosts of avian influenza in Nigeria, we randomly collected cloaca swab specimens from 155 ducks at a live bird market (LBM) in Ibadan, southwest Nigeria, between July 2011 and July 2012.

Methods

The samples were analyzed by real-time reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation was carried out in embryonated chicken eggs. Partial sequencing of the antigenic cleavage site of the haemagglutinin (HA) gene was performed, multiple sequence alignment was carried out using ClustalW, and a phylogenetic tree was constructed using the neighbor joining method.

Results

Twenty (13%) of the 155 samples were positive for avian influenza subtype H5N2 by real-time RT-PCR and three isolates were obtained from embryonated chicken eggs. Partial sequencing of the amino acid cleavage site of the HA genes of two isolates corresponded to a PQRETGL*F sequence that is common in low pathogenic avian influenza (LPAI). Phylogenetically, the HA genes of the two influenza viruses are monophyletic and clustered with H5N2 viruses detected in wild ducks from Africa.

Conclusion

The occurrence of LPAI in domestic ducks in Nigeria underscores the importance of continuous surveillance and monitoring of the virus (in a country that is considered to be free of avian influenza) in order to prevent the emergence of virulent strains that may spread to commercial poultry and humans.

【 授权许可】

   
2014 Coker et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Fusaro A, Joannis T, Monne I, Salviato A, Yakubu B, Meseko C, Fassina S, Capua I, Cattoli G: Introduction into Nigeria of a distinct genotype of avian influenza virus (H5N1). Emerg Infects Dis 2009, 15(3):445-447.
• [2]Oladokun AT, Meseko CA, Ighodalo E, John B, Ekong PS: Effect of intervention on the control of highly pathogenic avian influenza in Nigeria. Pan Afr Med J 2012, 13:14. PMC 3527058
• [3]World Health Organization: Influenza at the human-animal interface. http://www.who.int/influenza/human_animal/about/ent webcite Feb. 2012 (Accessed 20th February 2013)
• [4]Halvorson D, Karunakaram D, Senne D, Bailey C, Abraham A, Hinshaw V, Newman J: Epizootiology of Avian Influenza- simultaneous monitoring of sentinel ducks and turkeys in Minnesota. Avian Dis 1983, 27(1):77-85.
• [5]Meseko CA, Oladokun AT, Shehu B: An outbreak of Highly Pathogenic Avian Influenza (HPAI) in a mixed farm by the introduction of a waterfowl. Niger Vet J 2007, 28(3):67-69.
• [6]Ip HS, Dusek RJ, Heisey DM: The effect of swab sample choice on the detection of avian influenza in apparently healthy wild ducks. Avian Dis 2012, 56(1):114-119.
• [7]Ducatez MF, Hause B, Stigger-Rosser E, Darnell D, Corzo C, Juleen K, Simonson R, Brockwell-Staats C, Rubrum A, Wang D, Webb A, Crumpton J, Lowe J, Webby RJ: Multiple reassortment between pandemic (H1N1) 2009 and endemic influenza viruses in pigs. Emerg Infect Dis 2011, 17:1624-1629.
• [8]Gohrbandt S, Veits J, Hundt J, Bogs J, Breithaupt A, Teifke JP, Weber S, Mettenleiter TC, Stech J: Amino acid adjacent to haemagglutination site are relevant for virulence of avian influenza subtype H5. J Gen Virol 2011, 92:51-59.
• [9]Bao Y, Bolotov P, Dernovoy D, Kiryutin B, Zaslavsky L, Tatusova T, Ostell J, Lipman D: The influenza virus resource at the national center for biotechnology information. J Virol 2008, 82(2):596-601.
• [10]Alexander DJ: A review of avian influenza in different bird species. Vet Microbiol 2000, 74(1–2):3-13.
• [11]Henning J, Bett B, Okike I, Abdu P, Perry B: Incidence of highly pathogenic avian influenza H5N1 in Nigeria, 2005–2008. Transbound Emerg Dis 2012, 60(3):222-230.
• [12]Gaidet N, Cattoli G, Hammoumi S, Newman SH, Hagemeijer W, Takekawa JY, Cappelle J, Dodman T, Joannis T, Gil P, Monne I, Fusaro A, Capua I, Manu S, Micheloni P, Ottosson U, Mshelbwala JH: Evidence of infection by H5N2 highly pathogenic avian influenza viruses in healthy wild waterfowl. PLoS Pathog 2008, 4(8):e1000127. doi:10.1371
• [13]World Organization for Animal Healthhttp://www.oie.int/animal-health-in-the-world/update-on-avian-influenza webcite 2012. (Accessed 20th February 2013)
• [14]Snoeck CJ, Adeyanju AT, De Landtsheer S, Ottosson U, Manu S, Hagemeijer W, Mundkur T, Muller CP: Reassortant low-pathogenic avian influenza H5N2 viruses in African wild birds. J Virol 2011, 92:1172-1183.
• [15]ProMEDMAIL: International Society of Infectious Diseases. http://www.promedmail.org webcite (Accessed 10th March 2013)
• [16]World Organisation for Animal Health (OIE): World Animal Health Information Database, Weekly Disease Information 2013; 26 (11). http://www.oie.int/wahis_2/public/wahid.php/Diseaseinformation/reportarchive webcite (Accessed 11th July, 2014)
• [17]World Health Organization: Global Alert and Response (GAR): Human Infection with Influenza A (H7N9) Virus in China. Geneva, Switzerland; 2013. http://www.who.int/csr/don/2013_04_01/en/index.html webcite . (Accessed 20th May 2013)
• [18]Gao R, Cao B, Hu Y, Feng Z, Wang D, Hu W, Chen J, Jie Z, Qiu H, Xu H, Xu X, Lu H, Zhu W, Gao Z, Xiang N, Shen Y, He Z, Gu Y, Zhang Z, Yang Y, Zhao X, Zhou L, Li X, Zou S, Zhang Y, Li X, Yang L, Guo J, Dong J, Li Q, et al.: Human infection with a novel avian-origin influenza A (H7N9) virus. N Engl J Med 2013. doi:10.1056/NEJMoa1304459
• [19]Horimoto T, Kawaoka Y: Influenza: lessons from past pandemics, warning from current incidents. Nat Rev Microbiol 2005, 3:597.