Histopathological and ultra structural effects of nanoparticles on rat testis following 90 days (Chronic study) of repeated oral administration

Journal of Nanobiotechnology

DS Joshi³  Geeta Vanage⁵  Ujjwala Maheswari¹  Ipseeta R Mohanty⁴  Dipty Singh⁵  Himanshu Gupta³  Mansee Thakur² 

[1] Department Pathology, MGM Medical College, MGMIHS, Sector-1, Kamothe, Navi Mumbai, Maharashtra, India;MGMCET & Departments of Medical Biotechnology, Central Research Laboratory, MGMIHS Sector-1, Kamothe, Navi Mumbai, Maharashtra, India;Departments of Medical Genetics, Central Research Laboratory, MGMIHS Sector-1, Kamothe, Navi Mumbai, Maharashtra, India;Department of Pharmacology, MGM Medical College, MGMIHS, Sector-1, Kamothe, Navi Mumbai, Maharashtra, India;National Center for Preclinical Reproductive and Genetic Toxicology (NIRRH), National Institute for Research in Reproductive Health (ICMR), Jehangir Merwanji Street, Parel, Mumbai, Maharashtra, India

关键词: Chronic study;    Toxicity;    Distribution;    Oral exposure;    Nanoparticles;   

Others  :  1139423
DOI  :  10.1186/s12951-014-0042-8

 received in 2014-08-13, accepted in 2014-10-08,  发布年份 2014

【 摘 要 】

Background

Nanoparticles (Ag NPs) have recently received much attention for their possible applications in biotechnology and biomedical. However, little is known about the toxicity in reproductive organs of animal model following exposure to nanoparticles.

Objective

This study therefore, tried to examine the effects of nanoparticles with a diameter range of 5-20 nm on the histology of the testis of wistar rats and correlate it with Transmission Electron Microscopy results.

Materials and methods

Sixteen wistar rats were randomly divided into two groups of 8 rats each. Each group received the following via gavage technique for 90 days: Control Group (Group-1)-tap water; Experimental group (Group 2) - nanoparticles (20ug/kg/day). After ninety days (chronic study), rats were sacrificed and testis tissues was processed for histology and transmission electron microscopic study.

Results

There was significant difference between the observations of group-1 and group 2. The changes observed in the testis were disarray of the spermatogenic cells and disorientation of the testis. These changes were observed to have been disappearing from normal histological features. Detailed structural damages were observed with TEM analysis, such as depletion of germ cells, germinal cells necrosis, especially in spermatogonia and Leydig cells had an abnormal fibroblast-like appearance, abnormal space between neighboring sertoli cells, mitochondria, lost cristae and vacuolated (none energized) with those animals exposed to nanoparticles.

Conclusion

It seems that nanoparticles have acute and significant effects on spermatogenesis and number of spermatogenic cells. More experimental investigations are necessary to elucidate better conclusion regarding the safety of nanoparticles on male reproduction system.

【 授权许可】

   
2014 Thakur et al.; licensee BioMed Central Ltd.

【 图 表 】

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