期刊论文详细信息
Journal of Animal Science and Biotechnology
Effect of dietary stable isotopic ratios of carbon and nitrogen on the extent of their incorporation into tissues of rats
Jingdong Yin1  Boyang Yu1  Bing Dong1  Tingting Ju1  Wentao Lv1 
[1] State Key Laboratory of Animal Nutrition, China Agricultural University, No.2 West Road Yuanmingyuan, 100193, Beijing, China
关键词: Stable isotopes;    Nitrogen;    Diet;    Carbon;   
Others  :  814195
DOI  :  10.1186/2049-1891-3-14
 received in 2012-03-26, accepted in 2012-05-09,  发布年份 2012
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【 摘 要 】

This study was conducted to investigate the effect of different dietary ratios of 13 C to 12 C or 15 N to 14 N on their relative incorporation into tissues. Eighty male rats were used in two 21-day feeding trials in which they were fed diets with either high δ13C levels (δ13C = −13.89‰ and δ15N = 2.37‰ in experiment 1 and δ13C = −19.34‰ and δ15N = 4.73‰ in experiment 2) or low δ13C levels (δ13C = −17.90‰ and δ15N = 3.08‰ in experiment 1 and δ13C = −21.76‰ and δ15N = 0.53‰ in experiment 2), meanwhile, the dietary δ15N levels were designed to two ranks. Blood, liver, adipose and muscle tissues were collected on day 0, 3, 7, 14, and 21 for determination of 13 C, 12 C, 15 N and 14 N isotopes. Rat growth rate, antioxidant capacity and metabolic parameters were also assessed. The results indicate that adipose tissue tend to deplete 13 C before the stable isotopic ratios achieved final equilibrium. Therefore, feeds with different isotopic signatures had different incorporation rates into tissues. Low dietary 13 C levels decreased tissue δ13C values whereas high dietary 13 C levels did not alter tissue δ13C values during the 21-d experiment. Blood δ15N values were a reliable parameter in assessing the relative contribution of dietary nitrogen to tissues. This study revealed a relationship between dietary isotopic signatures and their incorporation rates into rat tissues. However, more studies are needed to illustrate the mechanism through which dietary isotopic ratios influence the extent of isotopic incorporation into the tissues.

【 授权许可】

   
2012 Lv et al.; licensee BioMed Central Ltd.

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