Pretreatment of garden biomass by alkali-assisted ultrasonication: effects on enzymatic hydrolysis and ultrastructural changes

Journal of Environmental Health Science Engineering

Satish Wate¹  Duraisamy Anand¹  Atul Narayan Vaidya¹  SPM Prince William¹  Jagdish Gabhane¹ 

[1] Solid and Hazardous Waste Management Division, National Environmental Engineering Research Institute, Nehru marg, Nagpur, Maharashtra, India

关键词: Ultra structural changes;    Cellulosic ethanol;    Enzymatic hydrolysis;    Garden biomass;    Alkali pretreatment;    Ultrasonication;   

Others  :  804846
DOI  :  10.1186/2052-336X-12-76

 received in 2013-05-09, accepted in 2014-04-12,  发布年份 2014

【 摘 要 】

The present investigation aims at studying the effectiveness of alkali-assisted ultrasonication on pretreatment of garden biomass (GB). Dry and powdered GB suspended in 1% NaOH was ultrasonicated for 15, 30 and 60 minutes at a frequency of 25 KHZ. The mode of action and effectiveness of alkali-assisted ultrasonication on GB was established through microscopic, scanning electron microscopic and X-ray diffraction studies. A perusal of results showed that alkali-assisted ultrasonication led to fibrillation of GB which ultimately facilitated enzymatic hydrolysis. The results also indicated that alkali-assisted ultrasonication is an efficient means of pretreatment of GB at moderate (45-50°C) working temperature and low (1%) concentration of alkali. The yield of reducing sugar after enzymatic hydrolysis increased almost six times as compared to control due to alkali-assisted ultrasonication.

【 授权许可】

   
2014 Gabhane et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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