Inhaled corticosteroid use is associated with increased circulating T regulatory cells in children with asthma

Clinical and Molecular Allergy

Christine M Seroogy⁵  James E Gern²  Robert F Lemanske²  Lisa Salazar⁵  Tressa Pappas⁵  Douglas DaSilva⁵  Christopher Tisler⁵  Kathy A Roberg⁵  Ronald Gangnon⁴  Michael D Evans¹  Kristjan Burmeister²  Paul Dahlberg²  Anne Marie Singh³ 

[1] Departments of Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI, USA;Departments of Medicine, University of Wisconsin, Madison, WI, USA;Northwestern Feinberg School of Medicine, Chicago, IL, USA;Departments of Population Health Sciences, University of Wisconsin, Madison, WI, USA;Departments of Pediatrics, University of Wisconsin, 1111 Highland Avenue, 4139 WIMR, Madison, WI 53705-2275, USA

关键词: T regulatory cell;    Inhaled corticosteroids;    Foxp3;    CD127;    Asthma;   

Others  :  790520
DOI  :  10.1186/1476-7961-11-1

 received in 2012-10-24, accepted in 2013-01-22,  发布年份 2013

【 摘 要 】

Background

T regulatory (Treg) cells are important in balancing immune responses and dysregulation of Treg cells has been implicated in the pathogenesis of multiple disease states including asthma. In this study, our primary aim was to determine Treg cell frequency in the peripheral blood of children with and without asthma. The secondary aim was to explore the association between Treg cell frequency with allergen sensitization, disease severity and medication use.

Methods

Peripheral blood mononuclear cells from healthy control subjects (N = 93) and asthmatic children of varying disease severity (N = 66) were characterized by multi-parameter flow cytometry.

Results

Our findings demonstrate that children with asthma had a significantly increased frequency of Treg cells compared to children without asthma. Using a multivariate model, increased Treg cell frequency in children with asthma was most directly associated with inhaled corticosteroid use, and not asthma severity, allergic sensitization, or atopic status of the asthma.

Conclusion

We conclude that low dose, local airway administration of corticosteroids is sufficient to impact the frequency of Treg cells in the peripheral blood. These data highlight the importance of considering medication exposure when studying Treg cells and suggest inhaled corticosteroid use in asthmatics may improve disease control through increased Treg cell frequency.

【 授权许可】

   
2013 Singh et al; licensee BioMed Central Ltd.

【 图 表 】

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