Immobilized laccase mediated dye decolorization and transformation pathway of azo dye acid red 27

Journal of Environmental Health Science Engineering

Trichur Ramaswamy Sreekrishnan¹  Saroj Mishra¹  Meenu Chhabra² 

[1] Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz-Khas, New-Delhi 110016, India;Department of Biology, Indian Institute of Technology-Jodhpur, Jodhpur 342011, Rajasthan, India

关键词: Azo dye degradation;    Acid Red 27;    Laccase-mediator;    Polyvinylalcohol beads;    Immobilization;   

Others  :  1216381
DOI  :  10.1186/s40201-015-0192-0

 received in 2013-11-26, accepted in 2015-04-15,  发布年份 2015

【 摘 要 】

Background

Laccases have good potential as bioremediating agents and can be used continuously in the immobilized form like many other enzymes.

Methods

In the present study, laccase from Cyathus bulleri was immobilized by entrapment in Poly Vinyl Alcohol (PVA) beads cross-linked with either nitrate or boric acid. Immobilized laccase was used for dye decolorization in both batch and continuous mode employing a packed bed column. The products of degradation of dye Acid Red 27 were identified by LC MS/MS analysis.

Results

The method led to very effective (90%) laccase immobilization and also imparted significant stability to the enzyme (more than 70% after 5 months of storage at 4°C). In batch decolorization, 90-95% decolorization was achieved of the simulated dye effluent for up to 10–20 cycles. Continuous decolorization in a packed bed bioreactor led to nearly 90% decolorization for up to 5 days. The immobilized laccase was also effective in decolorization and degradation of Acid Red 27 in the presence of a mediator. Four products of degradation were identified by LC-MS/MS analysis.

Conclusions

The immobilized laccase in PVA-nitrate was concluded to be an effective agent in treatment of textile dye effluents.

【 授权许可】

   
2015 Chhabra et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

【 参考文献 】

• [1]Maciel MJM, Silva ACE, Ribeiro HCT. Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review. Electron J Biotechn. 2010; 13:6.
• [2]Gholami-Borujeni F, Mahvi AH, Naseri S, Faramarzi MA, Nabizadeh R, Alimohammadi M. Enzymatic Treatment and Detoxification of Acid Orange 7 from Textile Wastewater. Appl Biochem Biotechnol. 2011; 165:1274-84.
• [3]Durán N, Rosa MA, D’Annibale A, Gianfreda L. Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review. Enzyme Microb Tech. 2002; 31:907-31.
• [4]Fernández-Fernández M, Sanromán MA, Moldes D. Recent developments and applications of immobilized laccase. Biotechnol Adv. 2013; 31:1808-25.
• [5]Reyes P, Pickard MA, Vazquez-Duhalt R. Hydroxybenzotriazole increases the range of textile dyes decolorized by immobilized laccase. Biotechnol Lett. 1999; 21:875-80.
• [6]Peralta-Zamora P, Pereira CM, Tiburtius ERL, Moraes SG, Rosa MA, Minussi RC et al.. Decolorization of reactive dyes by immobilized laccase. Appl Catal B Environ. 2003; 42:131-44.
• [7]Kunamneni A, Ghazi I, Camarero S, Ballesteros A, Plou FJ, Alcalde M. Decolorization of synthetic dyes by laccase immobilized on epoxy-activated carriers. Process Biochem. 2008; 43:169-78.
• [8]Ping W, Xuerong F, Li C, Qiang W, Aihui Z. Decolorization of reactive dyes by laccase immobilized in alginate/gelatin blent with PEG. J Environ Sci. 2008; 20:1519-22.
• [9]Hu X, Wang P, Hwang HM. Oxidation of Anthracene by immobilized laccase from Tramates versicolor. Bioresour Technol. 2009; 100:4963-8.
• [10]Champagne PP, Ramsay JA. Dye decolorization and detoxification by laccase immobilized on porous glass beads. Bioresour Technol. 2010; 101:2230-5.
• [11]Osma JF, Toca-Herrera JL, Rodriquez-Couto S. Transformation pathway of Remazol Brilliant Blue R by immobilized laccase. Bioresour Technol. 2010; 101:8509-14.
• [12]Asgher M, Kamal S, Muhammad H, Iqbal N. Improvement of Catalytic Efficiency, Thermo-stability and Dye Decolorization Capability of Pleurotus ostreatus IBL-02 laccase by Hydrophobic Sol Gel Entrapment. Chem Cent. 2012; 6:110. BioMed Central Full Text
• [13]Palmieri G, Giardina P, Sannia G. Laccase-Mediated Remazol Brilliant Blue R Decolorization in a Fixed-Bed Bioreactor. Biotechnol Prog. 2005; 21:1436-41.
• [14]Salony, Mishra S, Bisaria VS. Production and characterization of laccase from Cyathus bulleri and its use in decolorization of recalcitracnt textile dyes. Appl Microbiol Biotechnol. 2006; 71:646-53.
• [15]Salony, Mishra S, Bisaria VS. Decolorization and detoxification of textile dyes and black liquor by laccase of Cyathus bulleri. J Sci Ind Res. 2007; 66:684-8.
• [16]Chhabra M, Mishra S, Sreekrishnan TR. Mediator assisted decolorization and detoxification of textile dyes/ dye mixture using Cyathus bulleri laccase. Appl Biochem Biotechnol. 2008; 151:587-98.
• [17]Chhabra M, Mishra S, Sreekrishnan TR. Laccase/mediator assisted degradation of triarylmethane dyes in a continuous membrane reactor. J Biotechnol. 2009; 143(1):69-78.
• [18]Peijs T, Van Vught RJM, Govaert LE. Mechanical properties of poly(vinylalcohol) fibres and composites. Composites. 1995; 26:83-90.
• [19]Yujian W, Xiaojuan Y, Wei T, Hongyu L. High-rate ferrous iron oxidation by immobilized Acidithiobacillus ferrooxidans with complex of PVA and sodium alginate. J Microbiol Methods. 2006; 68:212-7.
• [20]Gholami-Borujeni F, Mahvi AH, Naseri S, Faramarzi MA, Nabizadeh R, Alimohammadi M. Application of immobilized horseradish peroxidase for removal and detoxification of azo dye from aqueous solution. Res J Chem Environ. 2011; 15(2):217-22.
• [21]Chen KC, Lin YF. Immobilization of microorganisms or enzymes in polyvinyl alcohol beads. US patent. 1994; 5:290-693.
• [22]Chang CC, Tseng SK. Immobilization of Alcaligenes eutrophus using PVA crosslinked with sodium nitrate. Biotechnol Tech. 1998; 12:865-8.
• [23]Brandi P, D’Annibale A, Galli C, Gentili P, Pontes ASN. In search for practical advantages from the immobilisation of an enzyme: the case of laccase. J Mol Catal B: Enzym. 2006; 41:61-9.
• [24]Ahn MY, Dec J, Kim JE, Bollag JM. Treatment of 2,4-Dichlorophenol Polluted Soil with Free and Immobilized Laccase. J Environ Qual. 2002; 31:1509-15.
• [25]Liu Y, Zeng Z, Zeng G, Tang L, Pang Y, Li Z et al.. Immobilization of laccase on magnetic bimodal mesoporous carbon and the application in the removal of phenolic compounds. Bioresour Technol. 2012; 115:21-6.
• [26]Champagne PP, Ramsay JA. Reactive Blue 19 decolourization by laccase on silica beads. Appl Microbiol Biotechnol. 2007; 77:819-23.
• [27]Chivukula M, Renganathan V. Phenolic Azo Dye Oxidation by Laccase from Pyricularia oryzae. Appl Environ Microbiol. 1995; 61:4374-7.
• [28]Zille A, Gornacka B, Rehorek A, Cavaco-Paulo A. Degradation of Azo Dyes by Trametes villosa Laccase over Long Periods of Oxidative Conditions. Appl Environ Microbiol. 2005; 71:6711-8.
• [29]Hong Y, Guo J, Xu Z, Mo C, Xu M, Sun G. Reduction and partial degradation mechanisms of naphthylaminesulfonic azo dye amaranth by Shewanella decolorationis S12. Appl Microbiol Biotechnol. 2007; 75:647-54.