Genotyping of Coxiella burnetii from domestic ruminants and human in Hungary: indication of various genotypes

BMC Veterinary Research

Miklós Gyuranecz²  Paul S Keim¹  Eszter Balla³  Ádám Dán⁴  Alexandra Szigeti²  Talima Pearson¹  Heidie M Hornstra¹  Zsuzsa Kreizinger²  Kinga M Sulyok² 

[1] Center for Microbial Genetics and Genomics, Northern Arizona University, 1298 S. Knoles Drive Flagstaff, AZ 86011-4073, USA;Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungária körút 21 1143, Hungary;National Center for Epidemiology, Budapest, Gyáli út 2-6 1097, Hungary;Veterinary Diagnostic Directorate, National Food Chain Safety Office, Budapest, Tábornok utca 2 1143, Hungary

关键词: Hungary;    MST;    MLVA;    Genotyping;    Q fever;    Coxiella burnetii;   

Others  :  1119193
DOI  :  10.1186/1746-6148-10-107

 received in 2014-01-17, accepted in 2014-04-28,  发布年份 2014

【 摘 要 】

Background

Information about the genotypic characteristic of Coxiella burnetii from Hungary is lacking. The aim of this study is to describe the genetic diversity of C. burnetii in Hungary and compare genotypes with those found elsewhere. A total of 12 samples: (cattle, n = 6, sheep, n = 5 and human, n = 1) collected from across Hungary were studied by a 10-loci multispacer sequence typing (MST) and 6-loci multiple-locus variable-number of tandem repeat analysis (MLVA). Phylogenetic relationships among MST genotypes show how these Hungarian samples are related to others collected around the world.

Results

Three MST genotypes were identified: sequence type (ST) 20 has also been identified in ruminants from other European countries and the USA, ST28 was previously identified in Kazakhstan, and the proposed ST37 is novel. All MST genotypes yielded different MLVA genotypes and three different MLVA genotypes were identified within ST20 samples alone. Two novel MLVA types 0-9-5-5-6-2 (AG) and 0-8-4-5-6-2 (AF) (Ms23-Ms24-Ms27-Ms28-Ms33-Ms34) were defined in the ovine materials correlated with ST28 and ST37. Samples from different parts of the phylogenetic tree were associated with different hosts, suggesting host-specific adaptations.

Conclusions

Even with the limited number of samples analysed, this study revealed high genetic diversity among C. burnetii in Hungary. Understanding the background genetic diversity will be essential in identifying and controlling outbreaks.

【 授权许可】

   
2014 Sulyok et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Angelakis E, Raoult D: Emergence of Q fever. Iran J Publ Health 2011, 40:1-18.
• [2]Angelakis E, Raoult D: Q fever. Vet Microbiol 2010, 140:297-309.
• [3]Raoult D, Marrie T, Mege J: Natural history and pathophysiology of Q fever. Lancet Infect Dis 2005, 5:219-226.
• [4]Tilburg JJ, Melchers WJ, Pettersson AM, Rossen JW, Hermans MH, van Hannen EJ, Nabuurs-Franssen MH, de Vries M, Horrevorts AM, Klaassen CHW: Interlaboratory evaluation of different extraction and real-time PCR methods for detection of Coxiella burnetii DNA in serum. J Clin Microbiol 2010, 48:3923-3927.
• [5]Landais C, Fenollar F, Thuny F, Raoult D: From acute Q fever to endocarditis: serological follow-up strategy. Clin Infect Dis 2007, 44:1337-1340.
• [6]Farkas E, Gerő S, Takátsy G: A Q láz előfordulása Magyarországon. Orv Hetil 1950, 91:717-718.
• [7]Romváry J: Incidence of Q-fever in a cattle herd. Magy Állatorv Lapja 1957, 12:25-27.
• [8]Gyuranecz M, Dénes B, Hornok S, Kovács P, Horváth G, Jurkovich V, Varga T, Hajtós I, Szabó R, Magyar T, Vass N, Hofmann-Lehmann R, Erdélyi K, Bhide M, Dán Á: Prevalence of Coxiella burnetii in Hungary: screening of dairy cows, sheep, commercial milk samples, and ticks. Vector Borne Zoonotic Dis 2012, 12:650-653.
• [9]Guatteo R, Seegers H, Taurel AF, Joly A, Beaudeau F: Prevalence of Coxiella burnetii infection in domestic ruminants: a critical review. Vet Microbiol 2011, 149:1-16.
• [10]Jäger C, Willems H, Thiele D, Baljer G: Molecular characterization of Coxiella burnetii isolates. Epidemiol Infect 1998, 120:157-164.
• [11]Hendrix L, Samuel J, Mallavia L: Differentiation of Coxiella burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE. J Gen Microbiol 1991, 137:269-276.
• [12]Stein A, Saunders NA, Taylor AG, Raoult D: Phylogenic homogeneity of Coxiella burnetii strains as determinated by 16S ribosomal RNA sequencing. FEMS Microbiol Lett 1993, 113:339-344.
• [13]Nguyen SV, Hirai K: Differentiation of Coxiella burnetii isolates by sequence determination and PCR-restriction fragment length polymorphism analysis of isocitrate dehydrogenase gene. FEMS Microbiol Lett 1999, 180:249-254.
• [14]Zhang GQ, To H, Yamaguchi T, Fukushi H, Hirai K: Differentiation of Coxiella burnetii by sequence analysis of the gene (com1) encoding a 27-kDa outer membrane protein. Microbiol Immunol 1997, 41:871-877.
• [15]Sekeyová Z, Roux V, Raoult D: Intraspecies diversity of Coxiella burnetii as revealed by com1 and mucZ sequence comparison. FEMS Microbiol Lett 1999, 180:61-67.
• [16]Massung RF, Cutler SJ, Frangoulidis D: Molecular typing of Coxiella burnetii (Q fever). In Coxiella burnetii: Recent advances and new perspectives in research of the Q fever bacterium. Volume 984. Edited by Toman R, Heinzen RA, Samuel JE, Mege J-L. Dordrecht: Springer Science + Business Media; 2012:381-396.
• [17]Glazunova O, Roux V, Freylikman O, Sekeyova Z, Fournous G, Tyczka J, Tokarevich N, Kovacova E, Marrie TJ, Raoult D: Coxiella burnetii genotyping. Emerg Infect Dis 2005, 11:1211-1217.
• [18]Arricau-Bouvery N, Hauck Y, Bejaoui A, Frangoulidis D, Bodier CC, Souriau A, Meyer H, Neubauer H, Rodolakis A, Vergnaud G: Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing. BMC Microbiol 2006, 6:38. BioMed Central Full Text
• [19]Svraka S, Toman R, Skultety L, Slaba K, Homan WL: Estabilishment of a genotyping scheme for Coxiella burnetii. FEMS Microbiol Lett 2006, 254:268-274.
• [20]Multi Spacers Typing - Coxiella Database. [http://ifr48.timone.univ-mrs.fr/MST_Coxiella/mst/ webcite]
• [21]Tilburg JJ, Roest HJ, Nabuurs-Franssen MH, Horrevorts AM, Klaassen CH: Genotyping reveals the presence of a predominant genotype of Coxiella burnetii in consumer milk products. J Clin Microbiol 2012, 50:2156-2158.
• [22]Loftis AD, Reeves WK, Szumlas DE, Abbassy MM, Helmy IM, Moriarity JR, Dasch GA: Surveillance of Egyptian fleas for agents of public health significance: Anaplasma, Bartonella, Coxiella, Ehrlichia, Rickettsia and Yersinia pestis. Am J Trop Med Hyg 2006, 75:41-48.
• [23]d-maps.com. [http://d-maps.com/carte.php?num_car=23243&lang=en webcite]
• [24]Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp 1999, 41:95-98.
• [25]Mediannikov O, Fenollar F, Socolovschi C, Diatta G, Bassene H, Molez J-F, Sokhna C, Trape J-F, Raoult D: Coxiella burnetii in humans and ticks in rural Senegal. PLoS Negl Trop Dis 2010, 4:e654.
• [26]Hornstra HM, Priestley RA, Georgia SM, Kachur S, Birdsell DN, Hilsabeck R, Gates LT, Samuel JE, Heinzen RA, Kersh GJ, Keim P, Massung RF, Pearson T: Rapid Typing of Coxiella burnetii. PLoS ONE 2011, 6:e26201.
• [27]Tilburg JJ, Rossen JWA, van Hannen EJ, Melchers WJ, Hermans MH, van de Bovenkamp J, Roest HJ, de Bruin A, Nabuurs-Franssen MH, Horrevorts AM, Klaassen CH: Genotypic diversity of Coxiella burnetii in the 2007–2010 Q fever outbreak episodes in The Netherlands. J Clin Microbiol 2012, 50:1076-1078.
• [28]Santos AS, Tilburg JJ, Botelho A, Barahona MJ, Nuncio MS, Nabuurs-Franssen MH, Klaassen CH: Genotypic diversity of clinical Coxiella burnetii isolates from Portugal based on MST and MLVA typing. Int J Med Microbiol 2012, 302:253-256.
• [29]Astobiza I, Tilburg JJ, Pinero A, Hurtado A, Garcia-Pérez AL, Nabuurs-Franssen MH, Klaassen CH: Genotyping of Coxiella burnetii from domestic ruminants in northern Spain. BMC Vet Res 2012, 8:241. BioMed Central Full Text
• [30]Klaassen CH, Nabuurs-Franssen MH, Tilburg JJ, Hamans MA, Horrevorts AM: Multigenotype Q fever outbreak, the Netherlands. Emerg Infect Dis 2009, 15:613-614.
• [31]Dryad [https://datadryad.org/ webcite, doi:10.5061/dryad.rc8q4]
• [32]Coxiella burnetii Cooperative MLVA database. [http://mlva.u-psud.fr/mlvav4/genotyping/ webcite]
• [33]Pearson T, Hornstra HM, Sahl JW, Schaack S, Schupp JM, Beckstrom-Sternberg SM, O’Neill MW, Priestley RA, Champion MD, Beckstrom-Sternberg JS, Kersh GJ, Samuel JE, Massung RF, Keim P: When outgroups fail; phylogenomics of rooting the emerging pathogen, Coxiella burnetii. Syst Biol 2013, 62:752-762.
• [34]Pearson T, Okinaka RT, Foster JT, Keim P: Phylogenetic understanding of clonal populations in an era of whole genome sequencing. Infect Genet Evol 2009, 2009(9):1010-1019.
• [35]Tilburg JJ, Roest HJ, Buffet S, Nabuurs-Franssen MH, Horrevorts AM, Raoult D, Klaassen CH: Epidemic genotype of Coxiella burnetii among goats, sheep, and humans in the Netherlands. Emerg Infect Dis 2012, 18:887-889.
• [36]Reichel R, Mearns R, Brunton L, Jones R, Horigan M, Vipond R, Vincent G, Evans S: Description of a Coxiella burnetii abortion outbreak in a dairy goat herd, and associated serology, PCR and genotyping results. Res Vet Sci 2012, 93:1217-1224.
• [37]Chmielewski T, Sidi-Boumedine K, Duquesne V, Podsiadly E, Thiery R, Tylewska-Wierzbanowska S: Molecular epidemiology of Q fever in Poland. Pol J Mic 2009, 58:9-13.
• [38]Mahamat A, Edouard S, Demar M, Abboud P, Patrice JY, La Scola B, Okzande A, Djossou F, Raoult D: Unique clone of Coxiella burnetii causing severe Q fever, French Guiana. Emerg Infect Diseases 2013, 19:1102.
• [39]Pearson T, Hornstra HM, Hilsabeck R, Gates LT, Olivas SM, Birdsell D, Hall C, German S, Cook JM, Seymour ML, Priestley R, Kondas AV, Friedman CL, Price EP, Schupp J, Liu C, Price LB, Massung RF, Kersh GJ, Keim P: High prevalence and two dominant host-specific genotypes of Coxiella burnetii in U.S. milk. BMC Microbiol 2014, 14:41. BioMed Central Full Text