MMP2 and MMP7 at the invasive front of gastric cancer are not associated with mTOR expression

Diagnostic Pathology

Peter Malfertheiner²  Michael Vieth¹  Elizabeth Bird-Lieberman³  Frank Meyer⁵  Doerthe Jechorek¹  Michael Selgrad²  Thomas Wex⁴  Alexander Link²  Cosima Langner²  Tina Seidel²  Jan Bornschein² 

[1] Institute for Pathology, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, Germany;Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, Germany;Translational Gastroenterology Unit, Experimental Medicine Division, Nuffield Department of Medicine, University of Oxford, Oxford, UK;Department of Molecular Genetics, Medical Laboratory for Clinical Chemistry, Microbiology and Infectious Diseases, Am Neustädter Feld 47, Magdeburg 39124, Germany;Department for General, Visceral and Vascular Surgery, Otto-von-Guericke University, Leipziger Str. 44, Magdeburg 39120, Germany

关键词: MMP9;    MMP7;    MMP2;    mTOR;    Gastric cancer;   

Others  :  1235139
DOI  :  10.1186/s13000-015-0449-z

 received in 2015-03-09, accepted in 2015-12-05,  发布年份 2015

【 摘 要 】

Background

Regulation of MMP expression by activation of mTOR signalling has been demonstrated for several tumor types, but has thus far not been confirmed in gastric cancer.

Findings

The study compromised 128 patients who underwent gastric resection for cancer (66.4 % male; 86 intestinal, 42 diffuse type). Immunohistochemical staining of MMPs was performed to analyse the topographical pattern of MMP expression at the tumor center and the invasive front, respectively. MMP2 showed higher expression at the invasive front compared to the tumor center, whereas MMP7 staining scores were higher in the tumor center, and there was no difference for MMP9. The expression of p-mTOR was higher in the tumor center than at the invasive front, with a similar trend for mTOR. For intestinal type gastric cancer there was a weak correlation of MMP9 with expression of mTOR in the tumor center. Otherwise, there was no correlation of the MMPs with mTOR. By treatment of MKN45 gastric cancer cells with rapamycin, a reduction of p-mTOR in the Western blot was achieved; however, expression of MMPs remained unaffected.

Conclusions

Expression of MMP2 and MMP7 in gastric cancer is not associated with mTOR, MMP9 expression might be related to mTOR signalling in a subset of tumors.

【 授权许可】

   
2015 Bornschein et al.

【 图 表 】

Fig. 1.

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