Effect of Matrine on HPAC cell migration by down-regulating the expression of MT1-MMP via Wnt signaling

Cancer Cell International

Guohua Song³  Jianguo Wang³  Song Meng³  Lei Yuan³  Zhanyu Bei¹  Xianjia Li³  Li Yin³  Wei Wan³  Junping Xiong³  Fazhang Zou²  Yongchao Ma² 

[1] Chengde Nursing Vocational College, Chengde, China;Key lab, of BioMedicine of Luohe City, Luohe, China;Luohe Medical College, Daxue Road, #148, Luohe City 462000, Henan Province, People’s Republic of China

关键词: Human pancreatic cancer;    Wnt;    MT1-MMP;    Cell migration;    Matrine;   

Others  :  1219347
DOI  :  10.1186/s12935-015-0210-4

 received in 2014-10-02, accepted in 2015-06-01,  发布年份 2015

【 摘 要 】

Aim

This study sought to explore the exact mechanism of Matrine inhibited migration and invasion of human pancreatic cancer cells.

Methods

HPAC or Capan-1 cells were cultured in completed RPMI-1640 medium, contained with 50 μg/ml Matrine or 0.05 μg/ml docetaxel, respectively. Cell viability was evaluated by spectrophotometric analysis using MTT assay. Wound healing assay and transwell approach were used to detect the effects of Matrine on HPAC cell migration and invasion. Western Blot and RT-PCR were performed to detect the expressions of MT1-MMP, Wnt and β-Catenin. CHIP assay was used to detect whether the MT1-MMP transcription activity correlated with Wnt signaling pathway.

Results

MTT results indicated that cell proliferration was inhibited by Matrine at a range of concentrations, especially at high dose. We further found that Matrine treatment significantly induced cell migration and invasion decreased. Interestingly, the expression of MT1-MMP decreased evidently upon Matrine treatment, paralleled with the expressions of Wnt and β-Catenin detected by Western Blot and RT-PCR assay. Further analysis of MT1-MMP transcription activity revealed that Matrine reduced the expression of MT1-MMP mediated by Wnt signaling pathway.

Conclusion

Matrine play a vital role in inhibiting HPAC cellular migration and invasion through down-regulating the expression of MT1-MMP via Wnt signaling pathway.

【 授权许可】

   
2015 Ma et al.

【 图 表 】

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