期刊论文详细信息
Lipids in Health and Disease
Liver uptake of gold nanoparticles after intraperitoneal administration in vivo: A fluorescence study
Mohsen Mahmoud Mady1  Mohamed Anwar K Abdelhalim2 
[1] Biophysics Department, Faculty of Science, Cairo University, 12613 Giza, Egypt;Department of Physics and Astronomy, College of Science, King Saud University, P.O. 2455, Riyadth 11451, Saudi Arabia
关键词: fluorescence spectroscopy;    liver tissue;    time-dependent effects;    sizes;    gold nanoparticles;   
Others  :  1212460
DOI  :  10.1186/1476-511X-10-195
 received in 2011-09-27, accepted in 2011-10-31,  发布年份 2011
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【 摘 要 】

Background

One particularly exciting field of research involves the use of gold nanoparticles (GNPs) in the detection and treatment of cancer cells in the liver. The detection and treatment of cancer is an area in which the light absorption and emission characteristics of GNPs have become useful. Currently, there are no data available regarding the fluorescence spectra or in vivo accumulation of nanoparticles (NPs) in rat liver after repeated administration. In an attempt to characterise the potential toxicity or hazards of GNPs in therapeutic or diagnostic use, the present study measured fluorescence spectra, bioaccumulation and toxic effects of GNPs at 3 and 7 days following intraperitoneal administration of a 50 μl/day dose of 10, 20 or 50 nm GNPs in rats.

Methods

The experimental rats were divided into one normal group (Ng) and six experimental groups (G1A, G1B, G2A, G2B, G3A and G3B; G1: 20 nm; G2: 10 nm; G3: 50 nm; A: infusion of GNPs for 3 days; B: infusion of GNPs for 7 days). A 50 μl dose of GNPs (0.1% Au by volume) was administered to the animals via intraperitoneal injection, and fluorescence measurements were used to identify the toxicity and tissue distribution of GNPs in vivo. Seventy healthy male Wistar-Kyoto rats were exposed to GNPs, and tissue distribution and toxicity were evaluated after 3 or 7 days of repeated exposure.

Results

After administration of 10 and 20 nm GNPs into the experimental rats, two fluorescence peaks were observed at 438 nm and 487 nm in the digested liver tissue. The fluorescence intensity for 10 and 20 nm GNPs (both first and second peaks) increased with the infusion time of GNPs in test rats compared to normal rats. The position of the first peak was similar for G1A, G2A, G1B, G2B, G3B and the normal (438 nm); that for G3A was shifted to a longer wavelength (444 nm) compared to the normal. The position of the second peak was similar for G1A, G1B, G2A, G2B and the control (487 nm), while it was shifted to a shorter wavelength for G3A (483 nm) and G3B (483 nm). The fluorescence intensity of the first and second peaks increased for G1A, G2A, G1B and G2B, while it decreased for G3A and G3B compared to the control.

Conclusions

The fluorescence intensity of GNPs varied with the number, size and shape of particles and with the ratio of surface area to volume in a given sample. Fluorescence intensity changes during infusion depended on the size and shape of GNPs, with smaller particles experiencing larger changes during the infusion time in addition to the quenching produced by the larger GNPs. It is likely that smaller particles, which have a much higher ratio of surface area to volume compared to larger particles, are more prone to aggregation and surface interaction with biological components. This study suggests that fluorescence intensity can be used to evaluate bioaccumulation and the toxicity of gold nanoparticles in rats.

【 授权许可】

   
2011 Abdelhalim and Mady; licensee BioMed Central Ltd.

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【 参考文献 】
  • [1]Caruthers SD, Wickline SA, Lanza GM: Nanotechnological applications in medicine. Curr Opin Biotechno 2007, 18:26-30.
  • [2]Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann M: In Vitro Cytotoxicity of Nanoparticles in Mammalian Germline Stem Cells. Toxicol Sci 2005, 88:412-419.
  • [3]Oberdörster G, Maynard A, Donaldson K, Castranova V, Fitzpatrick J, Ausman K, Carter J, Kan B, Kreyling W, Lai D, Olin S, Monteiro-Riviere N, Warheit D: Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy. Particle and Fibre Toxicology 2005, 2:13-35.
  • [4]Foster W, Ruka M, Gareau P, et al.: Morphologic characteristics of endometriosis in the mouse model: application to toxicology. Can J Phsiol Pharmacol 1997, 75:1188-1196.
  • [5]Pissuwan D, Valenzuela SM, Cortie MB: Therapeutic possibilities of plasmonically heated gold nanoparticles. Trends Biotechno 2006, 24:62-67.
  • [6]Kogan MJ, Olmedo I, Hosta L, Guerrero AR, Cruz LJ, Albericio F: Peptides and metallic nanoparticles for biomedical applications. Nanomedicine 2007, 2:287-306.
  • [7]El-Sayed IH, Huang X, El-Sayed MA: Selective laser photo-thermal therapy of epithelial carcinoma using anti-EGFR antibody conjugated gold nanoparticles. Cancer Lett 2006, 239:129-135.
  • [8]Kogan MJ, Bastus NG, Amigo R, Grillo-Bosch D, Araya E, Turiel A, Labarta A, Giralt E, Puntes VF: Nanoparticle-mediated local and remote manipulation of protein aggregation. Nano Lett 2006, 6:110-115.
  • [9]Zharov VP, Mercer KE, Galitovskaya EN, Smeltzer MS: Photothermal nanotherapeutics and nanodiagnostics for selective killing of bacteria targeted with gold nanoparticles. Biophys J 2006, 90:619-627.
  • [10]Semmler-Behnke M, Kreyling WG, Lipka J, Fertsch S, Wenk A, Takenaka S, Schmid G, Brandau W: Biodistribution of 1.4- and 18-nm gold particles in rats. Small 2008, 4:2108-2111.
  • [11]Schmid G: Large clusters and colloids. Metals in the embryonic state. Chem Rev 1992, 92:1709.
  • [12]Jain P, El-Sayed I, El-Sayed M: Au nanoparticles target cancer. Nano Today 2007, 2:18-29.
  • [13]Tian ZQ, Bin R, Wu DY: Surface-Enhanced Raman Scattering: From Noble to Transition Metals and from Rough Surfaces to Ordered Nanostructures. J Phys Chem B 2002, 106:9463-9483.
  • [14]Kamat PV: Photophysical, Photochemical and Photocatalytic Aspects of Metal Nanoparticles. J Phys Chem B 2002, 106:7729-7744.
  • [15]Shipway AN, Eugenii K, Itamar W: Nanoparticle Arrays on Surfaces for Electronic, Optical, and Sensor Applications. Chem Phys Chem 2000, 1:18-52.
  • [16]Kuwahara Y, Yamada S: Facile Fabrication of Photoelectrochemical Assemblies Consisting of Gold Nanoparticles and a Tris(2, 2'-bipyridine)ruthenium(II)-Viologen Linked Thiol. Langmuir 2001, 17:5714-5716.
  • [17]Mirkin CA, Letsinger RL, Mucic RC, Storhoff JJ: A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 1996, 382:607-609.
  • [18]Huber M, Wei TF, Muller UR, Lefebvre PA, Marla SS, Bao YP: Gold nanoparticle probe-based gene expression analysis with unamplified total human RNA. Nucleic Acids Res 2004, 32:e137-e145.
  • [19]Wang X, Duan S, Geng B, Cui J, Yang Y: Schmeissneria: A missing link to angiosperms? BMC Evol Biology 2007, 7:1-13. BioMed Central Full Text
  • [20]Nel A, Xia T, Mädler L, Li N: Toxic Potential of Materials at the Nanolevel. Science 2006, 311:622-627.
  • [21]Liu WT: Nanoparticles and their biological and environmental applications. J Biosci Bioeng 2006, 102:1-7.
  • [22]Baptista P, Pereira E, Eaton P, Doria G, Miranda A, Gomes I, et al.: Gold nanoparticles for the development of clinical diagnosis methods. Anal Bioanal Chem 2008, 391:943-50.
  • [23]Hillyer JF, Albrecht RM: Gastrointestinal persorption and tissue distribution of differently sized colloidal gold nanoparticles. J Pharm Sci 2001, 90:1927-1936.
  • [24]Sonavane G, Tomoda K, Makino K: Biodistribution of colloidal gold nanoparticles after intravenous administration: effect of particle size. Colloids Surf B Biointerfaces 2008, 66:274-280.
  • [25]De Jong WH, Hagens WI, Krystek P, Burger MC, Sips AJ, Geertsma RE: Particle size-dependent organ distribution of gold nanoparticles after intravenous administration. Biomaterials 2008, 29:1912-1919.
  • [26]Hainfeld JF, Slatkin DN, Focella TM, Smilowitz HM: Gold nanoparticles: a new X-ray contrast agent. Brit J Radiol 2006, 79:248-253.
  • [27]Shrestha S, Yeung C, Nunnerley C, Tsang S: Comparison of morphology and electrical conductivity of various thin films containing nano-crystalline praseodymium oxide particles. Sens Actuators A Phys 2006, 136:191-8.
  • [28]Cho W-S, Cho M, Jeong J, Choi M, Han BS, Shin H-S, Hong J, Chung BH, Jeong J, Cho M-H: Size-dependent tissue kinetics of PEG-coated gold nanoparticles. Toxicology and Applied Pharmacology 2010, 245:116-123.
  • [29]Choi CJ, Anantharam V, Nathan J, Saetveit NJ, Houk RS, Arthi Kanthasamy A, Anumantha G, Kanthasamy AG: Normal Cellular Prion Protein Protects against Manganese-induced Oxidative Stress and Apoptotic Cell Death. Toxicol Sci 2007, 52:280-283.
  • [30]Gupta R: System behaviour of wood truss assemblies. Progress in Structural Engineering and Materials 2005, 7:183-193.
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