Alterative effects of an oral alginate extract on experimental rabbit osteoarthritis

Journal of Biomedical Science

Chien-Ho Chen⁴  Yung-Feng Lin⁴  Chian-Her Lee⁵  Yung-Hsiang Chang⁷  Suk Jung Oh¹  Kwang Yoon Kim¹  Jai Lan⁶  Tsuey-Ying Hsu⁶  Li-Fan Yao⁴  Chao-Wen Cheng²  Ming-Shium Hsieh³  Hsien-Tseng Lu⁵ 

[1] Ecobio Inc., Nonsan, Chungbuk, Korea;Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;Department of Orthopedics, En Chu Kong Hospital, New Taipei, Taiwan;School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, 250 Wu Xing St., Taipei 110, Taiwan;Department of Orthopedics, Taipei Medical University Hospital, Taipei, Taiwan;Mastervet International Marketing Limited, Taipei, Taiwan;School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan

关键词: CTX;    Aggrecan;    Matrix metalloproteinases;    Interleukin-1β;    Anterior cruciate ligament transection;    Osteoarthritis;   

Others  :  1232321
DOI  :  10.1186/s12929-015-0169-4

 received in 2015-06-08, accepted in 2015-07-21,  发布年份 2015

【 摘 要 】

Background

Osteoarthritis (OA) is a common joint disease that causes disabilities in elderly. However, few agents with high efficacy and low side effects have been developed to treat OA. In this study, we evaluated the effects of the alginate extract named CTX in OA cell and rabbit models.

Results

CTX was formulated by hydrolyzing sodium alginate polymers with alginate lyase and then mixing with pectin. HPLC was used to analyze the CTX content. Human chondrosarcoma SW1353 cells treated with interleukin-1β were used as OA model cells to investigate the effects of CTX on chondrocyte inflammation and anabolism. CTX at concentrations up to 1000 μg/ml exerted low cytotoxicity. It inhibited the gene expression of proinflammatory matrix metalloproteinases (MMPs) including MMP1, MMP3 and MMP13 in a dose-dependent manner and increased the mRNA level of aggrecan, the major proteoglycan in articular cartilage, at 1000 μg/ml. Thirteen-week-old New Zealand White rabbits underwent a surgical anterior cruciate ligament transection and were orally treated with normal saline, glucosamine or CTX for up to 7 weeks. Examinations of the rabbit femur and tibia samples demonstrated that the rabbits taking oral CTX at a dosage of 30 mg/kg/day suffered lesser degrees of articular stiffness and histological cartilage damage than the control rabbits.

Conclusions

The gene expression profiles in the cell and the examinations done on the rabbit cartilage suggest that the alginate extract CTX is a pharmaco-therapeutic agent applicable for OA therapy.

【 授权许可】

   
2015 Lu et al.

【 图 表 】

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