Immunogenicity and protective efficacy of Salmonella enterica serovar Pullorum pathogenicity island 2 mutant as a live attenuated vaccine candidate

BMC Veterinary Research

Xinan Jiao¹  Zhiming Pan¹  Shizhong Geng¹  Qiuchun Li¹  Lijuan Xu¹  Zhao Cheng¹  Junlei Yin¹ 

[1]Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, Jiangsu, P R China

关键词: live attenuated vaccine;    Salmonella pathogenicity island 2;    Pullorum disease;    Salmonella enterica serovar Pullorum;   

Others  :  1224422
DOI  :  10.1186/s12917-015-0497-3

 received in 2015-03-30, accepted in 2015-07-16,  发布年份 2015

【 摘 要 】

Background

Salmonella enterica serovar Pullorum (S. Pullorum) causes Pullorum disease (PD), a severe systemic disease of poultry and results in considerable economic losses in developing countries. In order to develop a safe and immunogenic vaccine, the immunogenicity and protective efficacy of S06004ΔSPI2, a Salmonella pathogenicity island 2 (SPI2) deleted mutant of S. Pullorum was evaluated in 2-day old chickens.

Results

Single intramuscular vaccination with S06004ΔSPI2 (2 × 10 ⁷  CFU) of chickens revealed no differences in body weight or clinical symptoms compared to control group. S06004ΔSPI2 bacteria can colonize and persistent in liver and spleen of vaccinated chickens approximately 14 days, and specific humoral and cellular immune responses were significantly induced. Vaccination of chickens offered efficient protection against S. Pullorum strain S06004 and S. Gallinarum strain SG9 challenge, respectively, at 10 days post vaccination (dpv) based on mortality and clinical symptoms compared to control group.

Conclusions

These findings suggest that S06004ΔSPI2 appears to be a highly immunogenic and efficient live attenuated vaccine candidate.

【 授权许可】

   
2015 Yin et al.

【 图 表 】

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