CpG sites associated with NRP1, NRXN2 and miR-29b-2 are hypomethylated in monocytes during ageing

Immunity & Ageing

Pärt Peterson²  Lili Milani¹  Andres Metspalu¹  Kai Kisand²  Mario Saare²  Maia Limbach²  Liina Tserel² 

[1] Estonian Genome Centre, University of Tartu, Tartu, Estonia;Molecular Pathology, Institute of Biomedicine and Translational Medicine, University of Tartu, 19 Ravila St, Tartu 50411, Estonia

关键词: Ageing;    DNA methylation;    Monocytes;   

Others  :  814129
DOI  :  10.1186/1742-4933-11-1

 received in 2013-09-06, accepted in 2013-12-20,  发布年份 2014

【 摘 要 】

Background

Ageing affects many components of the immune system, including innate immune cells like monocytes. They are important in the early response to pathogens and for their role to differentiate into macrophages and dendritic cells. Recent studies have revealed significant age-related changes in genomic DNA methylation in peripheral blood mononuclear cells, however information on epigenetic changes in specific leukocyte subsets is still lacking. Here, we aimed to analyse DNA methylation in purified monocyte populations from young and elderly individuals.

Findings

We analysed the methylation changes in monocytes purified from young and elderly individuals using the HumanMethylation450 BeadChip array. Interestingly, we found that among 26 differentially methylated CpG sites, the majority of sites were hypomethylated in elderly individuals. The most hypomethylated CpG sites were located in neuropilin 1 (NRP1; cg24892069) and neurexin 2 (NRXN2; cg27209729) genes, and upstream of miR-29b-2 gene (cg10501210). The age-related hypomethylation of these three sites was confirmed in a separate group of young and elderly individuals.

Conclusions

We identified significant age-related hypomethylation in human purified monocytes at CpG sites within the regions of NRP1, NRXN2 and miR-29b-2 genes.

【 授权许可】

   
2014 Tserel et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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