Effect of exercise intensity on weight changes and sexual hormones (androstenedione and free testosterone) in female rats with estradiol valerate-induced PCOS

Journal of Ovarian Research

Farzaneh Alipour³  Hojatolah Karimi Jashni¹  Maryamosadat Miri² 

[1] Department of Anatomy, Jahrom University of Medical Sciences, Jahrom, Iran;Exercise Physiology, Jahrom University of Medical Sciences, Jahrom, Fars, Iran;Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Fars, Iran

关键词: Free testosterone;    Androstenedione;    Weight change;    Exercise intensity;   

Others  :  801710
DOI  :  10.1186/1757-2215-7-37

 received in 2014-01-22, accepted in 2014-02-15,  发布年份 2014

【 摘 要 】

Introduction

Weight gain and fat accumulation are predisposing factors of PCOS. Life-style modification, including increasing physical activity, is the first line approach in managing PCOS. The objective of this study is to assess the effect of exercise intensity on weight changes, androstenedione and free testosterone level in female rats with estradiol valerate induced PCOS.

Method and materials

40 female Wistar rats were selected (180 ± 20 g). They had every 2 to 3 consecutive estrous cycles during 12 to 14 days. The study was approved by ethical committee of Jahrom University of Medical Sciences. The first two groups were divided into control (n = 10) and polycystic (n = 30) that were induced PCOS by estradiol valerate injection after 60 days. The polycystic groups were divided into three groups of sham (n = 10), experiment group with low-intensity exercise (pco + l.exe) (n = 10) and experiment group with moderate intensity exercise (pco + m.exe) (n = 10). Exercises were performed during 6 sessions of 60 minutes per week for 8 weeks. (Moderate intensity: 28 m/min-70%–75%VO2Max. Low intensity (20 m/min-50%–55%VO2Max) running at 0 slope, 1 h/day, 6 days/week). ANOVA and LSD test were used for data analysis.

Results

In the present study, no significant differences were found in the decrease of total weights of rats. And also androstenedione level changes in experiment groups were higher compared to control group but no significant differences were found, also free testosterone level was significantly higher than the observer group.

Conclusion

According to weight changes and sexual hormones (Free testosterone and androstenedione) exercise training especially with low intensity may improve symptoms of polycystic ovary syndrome.

【 授权许可】

   
2014 Miri et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

【 参考文献 】

• [1]Sarvari A, Naderi MM, Heidari M, Zarnani AH, Jeddi-Tehrani M, Sadeghi MR, Akhondi MM: Effect of environmental risk factor on human fertility. J Reprod Infertil 2010, 11(4):341-355.
• [2]Marcondes JAM, Hayashida SY, Bachega TASS: Hirsutismo & Síndromes dos ovários policísticos. In Endocrinologia. Edited by Mendonça BB, Maciel RMB, Saad M. São Paulo, Brazil: Atheneu; 2007:635-682.
• [3]Forozanfard F: Ovulation Step by Step. Kashan: Printing, Publishing Morsel; 2010:81-105.
• [4]Hatami H, Razavi SM, Eftekhar AH, Majlesi F, Sayed Nozadi M, Parizadeh SMJ: Text Book of Public Health. 1st edition. Tehran: Arjmand Publications; 2006. P. 1656 2
• [5]Hashemichashemi Z, Garavand N, Dehkordi KH, Ravasi A, Sardari M: Comparison of single bout of moderate intensity exercise on growth hormone active and inactive women. Jundishapur J Med 2011, 11(2):147-156.
• [6]Christopher N, Tymchuk Sheva B, Tessler R, Bernard J: Changes in sex hormone-binding globulin, insulin, and serum lipids in postmenopausal women on a low-fat, high-fiber diet combined with exercise. Nutr Cancer 2000, 38(2):158-162.
• [7]Franks S, Adams J, Mason H, Polson D: Ovulatory disorders in women with polycystic ovary syndrome. Clin Obstet Gynaecol 1985, 12(3):605-632.
• [8]Singh KB, Mahajan DK, Wortsman J: Effect of obesity on the clinical and hormonal characteristics of the polycystic ovary syndrome. J Reprod Med 1994, 39(10):805-808.
• [9]Hoeger M: Exercise therapy in polycystic ovary syndrome. Semin Reprod Med 2008, 26(1):93-100.
• [10]Palomba S, Giallauria F, Falbo A, Russo T, Oppedisano R, Tolino A, Colao A, Vigorito C, Zullo F, Orio F: Structured exercise training programme versus hypocaloric hyperproteic diet in obese polycystic ovary syndrome patients with anovulatory infertility: a 24-week pilot study. Hum Reprod 2008, 23:642-650.
• [11]Gilling-Smith C, Willis DS, Beard RW, Franks S: Hypersecretion of androstenedione by isolated thecal cells from polycystic ovaries. J Clin Endocrinol Metab 1994, 79:1158-116512.
• [12]Dunaif A, Segal KR, Futterweit W, Dobrjansky A: Profound peripheral insulin resistance, independent of obesity, in polycystic ovary syndrome. Diabetes 1989, 38(9):1165-1174.
• [13]dos Reis RM, Foss MC, de Moura MD, Ferriani RA, Silva de Sá MF: Insulin secretion in obese and non-obese women with polycystic ovary syndrome and its relationship with hyperandrogenism. Gynecol Endocrinol 1995, 9(1):45-50.
• [14]Moran LJ, Harrison CL, Hutchison SK, Stepto N, Strauss BJ, Teede HJ: Exercise decreases anti-mullerian hormone in anovulatoryoverwight women with polycystic ovary syndrome-a pilot study. Thieme eJournals 2011, 21989557. pubmed–as supplied by publisher
• [15]Wright CE, Zborowski JV, Talbott EO, McHugh-Pemu K, Youk A: Dietary intake, physical activity, and obesity in women with polycystic ovary syndrome. Int J Obes 2004, 28(8):1026-1032.
• [16]Moran LJ, Brinkworth G, Noakes M, Norman RJ: Effects of lifestyle modification in polycystic ovarian syndrome. Reprod BioMed Online 2006, 12(5):569-578. Article no. 2166
• [17]Pasquali R, Casimirri F, Balestra V, Flamia R, Melchionda N, Fabbri R, Barbara L: The relative contribution of androgens and insulin in determining abdominal fat distribution in 19 premenopausal women. J Endocrinol Invest 1991, 14:839.
• [18]Peiris AN, Sothmann MS, Aiman EJ, Kissebah AH: The relationship of insulin to sex hormone binding globulin: role of adiposity. Fertil Steril 1989, 52:69.
• [19]Consitt LA, Copeland JL, Tremblay MS: Hormone responses to resistance vs endurance exercise in premenopausal females. Can J Appl Physiol 2001, 26(6):574-587.
• [20]Holte J, Bergh T, Berne C, Lithell H: Serum lipoprotein lipid profile in women with the polycystic ovary syndrome: relation to anthropometric, endocrine and metabolic variables. Clin Endocrinol 1994, 41(4):463-471.
• [21]Acién P, Quereda F, Matallín P, Villarroya E, López-Fernández JA, Acién M, Mauri M, Alfayate R: Insulin, androgens, and obesity in women with and without polycystic ovary syndrome: a heterogeneous group of disorders. Fertil Steril 1999, 72(1):32-40.
• [22]Moran C, Renteria JL, Moran S, Herrera J, Gonzalez S, Bermudez JA: Obesity differentially affects serum levels of androstenedione and testosterone in polycystic ovary syndrome. Fertil Steril 2008, 90(6):2310-2317.