Pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells

Cancer Cell International

Lei Zhao³  Shu-Ling Zhang³  Ling-Sheng Kong²  Yang Liu²  Xi-Zhen Yu²  Gen-Hua Li²  Guang-Kui Han²  Ren-Ya Zhang¹  Chuan-Tao Yuan¹  Song Feng²  Ran Zhang²  Feng Jin² 

[1]Department of Pathology, Affiliated Hospital of Jining Medical University, Jining 272029, Shandong, PR China

[2]Department of Neurosurgery, Affiliated Hospital of Jining Medical University, and Shangdong Provincial Key Laboratory of Stem Cells and Neuro-oncology, Jining 272029, Shandong, PR China

[3]Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China

关键词: Transplanted tumor;    Animal model;    Cancer stem-like cell;    Glioblastoma;    CD133;   

Others  :  1219346
DOI  :  10.1186/s12935-015-0208-y

 received in 2014-05-11, accepted in 2015-05-22,  发布年份 2015

【 摘 要 】

Background

This study is to explore the pathological features of transplanted tumor established by CD133 positive TJ905 glioblastoma stem-like cells.

Methods

CD133 positive TJ905 glioma cells were separated by immunomagnetic beads to isolate glioma stem-like cells. TJ905 cells and stem-like cells were inoculated subcutaneously into the mice to establish model of transplanted tumor, respectively. Mice growing condition and behavior were observed. HE staining assay, immunohistochemical assay for GFAP, Ki-67 and Olig-2, and CD34 marked microvascular density (MVD) test were performed.

Results

The growing condition and behavior of mice in TJ905 stem cell group was more exaggerated and the models showed stronger malignant features pathologically than that in TJ905 cell group. Glial fibrillary acidic protein (GFAP) in TJ905 cell and stem-like cell group showed the transplanted tumor originated from astrocytes. Expression of Ki-67 and oligodendrocyte transcription factor-2 (Olig-2) in TJ905 stem cells was higher notably and CD34 expression in stem cell group was significantly higher than that in the other two groups.

Conclusions

Pathological features of transplanted tumor established by CD133 positive glioblastoma stem-like cells show more malignant. Use of TJ905 stem cells to establish transplanted tumor model in nude mice is excellent for glioma research.

【 授权许可】

   
2015 Jin et al.

【 图 表 】

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