Expression of the pair-rule gene homologs runt, Pax3/7, even-skipped-1 and even-skipped-2 during larval and juvenile development of the polychaete annelid Capitella teleta does not support a role in segmentation

EvoDevo

Néva P Meyer²  Gemma S Richards³  Emi Yamaguchi¹  Elaine C Seaver¹ 

[1] Kewalo Marine Laboratory, Pacific Biosciences Research Center, University of Hawaii, 41 Ahui Street, Honolulu, HI, USA;Biology Department, Clark University, Worcester, MA, USA;Sars International Centre for Marine Molecular Biology, Bergen, Norway

关键词: Segmentation;    Runt;    polychaete;    Pax3/7;    Eve;    Capitella;   

Others  :  807661
DOI  :  10.1186/2041-9139-3-8

 received in 2011-11-28, accepted in 2012-04-18,  发布年份 2012

【 摘 要 】

Background

Annelids and arthropods each possess a segmented body. Whether this similarity represents an evolutionary convergence or inheritance from a common segmented ancestor is the subject of ongoing investigation.

Methods

To investigate whether annelids and arthropods share molecular components that control segmentation, we isolated orthologs of the Drosophila melanogaster pair-rule genes, runt, paired (Pax3/7) and eve, from the polychaete annelid Capitella teleta and used whole mount in situ hybridization to characterize their expression patterns.

Results

When segments first appear, expression of the single C. teleta runt ortholog is only detected in the brain. Later, Ct-runt is expressed in the ventral nerve cord, foregut and hindgut. Analysis of Pax genes in the C. teleta genome reveals the presence of a single Pax3/7 ortholog. Ct-Pax3/7 is initially detected in the mid-body prior to segmentation, but is restricted to two longitudinal bands in the ventral ectoderm. Each of the two C. teleta eve orthologs has a unique and complex expression pattern, although there is partial overlap in several tissues. Prior to and during segment formation, Ct-eve1 and Ct-eve2 are both expressed in the bilaterial pair of mesoteloblasts, while Ct-eve1 is expressed in the descendant mesodermal band cells. At later stages, Ct-eve2 is expressed in the central and peripheral nervous system, and in mesoderm along the dorsal midline. In late stage larvae and adults, Ct-eve1 and Ct-eve2 are expressed in the posterior growth zone.

Conclusions

C. teleta eve, Pax3/7 and runt homologs all have distinct expression patterns and share expression domains with homologs from other bilaterians. None of the pair-rule orthologs examined in C. teleta exhibit segmental or pair-rule stripes of expression in the ectoderm or mesoderm, consistent with an independent origin of segmentation between annelids and arthropods.

【 授权许可】

   
2012 Seaver et al; licensee BioMed Central Ltd.

【 图 表 】

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