Laccase-catalyzed decolorization and detoxification of Acid Blue 92: statistical optimization, microtoxicity, kinetics, and energetics

Journal of Environmental Health Science Engineering

Mohammad Ali Faramarzi¹  Mohammad Reza Khoshayand²  Hamid Forootanfar³  Alieh Ameri⁵  Mehdi Mogharabi¹  Hamed Tahmasbi⁴  Shahla Rezaei⁴ 

[1] Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 1417614411, Iran;Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceuticals Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran 1417614411, Iran;Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran;Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran 1417614411, Iran;Department of Medicinal Chemistry, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

关键词: Decolorization;    Laccase;    Bioremediation;    Waste Treatment;    Optimization;    Enzyme Biocatalysis;   

Others  :  1175268
DOI  :  10.1186/s40201-015-0183-1

 received in 2014-11-14, accepted in 2015-04-07,  发布年份 2015

【 摘 要 】

Background

In recent years, enzymatic-assisted removal of hazardous dyes has been considered as an alternative and eco-friendly method compared to those of physicochemical techniques. The present study was designed in order to obtain the optimal condition for laccase-mediated (purified from the ascomycete Paraconiothyrium variabile) decolorization of Acid Blue 92; a monoazo dye, using response surface methodology (RSM). So, a D-optimal design with three variables, including pH, enzyme activity, and dye concentration, was applied to optimize the decolorization process. In addition, the kinetic and energetic parameters of the above mentioned enzymatic removal of Acid Blue 92 was investigated.

Results

Decolorization of Acid Blue 92 was maximally (94.1% ± 2.61) occurred at pH 8.0, laccase activity of 2.5 U/mL, and dye concentration of 75 mg/mL. The obtained results of kinetic and energetic studies introduced the laccase-catalyzed decolorization of Acid Blue 92 as an endothermic reaction (Ea, 39 kJ/mol; ΔS, 131 J/mol K; and ΔH, 40 kJ/mol) with K_m and V_max values of 0.48 mM and 227 mM/min mg, respectively. Furthermore, the results of microtoxicity study revealed that the toxicity of laccase-treated dye was significantly reduced compared to the untreated dye.

Conclusions

To sum up, the present investigation introduced the Paraconiothyrium variabile laccase as an efficient biocatalyst for decolorization of synthetic dye Acid Blue 92.

【 授权许可】

   
2015 Rezaei et al.; licensee BioMed Central.

【 图 表 】

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