BMC Veterinary Research | |
Orf virus interferes with MHC class I surface expression by targeting vesicular transport and Golgi | |
Hanns-Joachim Rziha1  Michael R Knittler1  Frederic Emschermann1  Jörg Rohde1  | |
[1] Friedrich-Loeffler-Institute, Federal Research Institute of Animal Health, Institute of Immunology, Greifswald-Insel Riems, Germany | |
关键词: Golgi apparatus; Immunomodulation; Subversion; MHC class I; Parapoxvirus; Orf virus; | |
Others : 1119773 DOI : 10.1186/1746-6148-8-114 |
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received in 2012-03-07, accepted in 2012-05-29, 发布年份 2012 | |
【 摘 要 】
Background
The Orf virus (ORFV), a zoonotic Parapoxvirus, causes pustular skin lesions in small ruminants (goat and sheep). Intriguingly, ORFV can repeatedly infect its host, despite the induction of a specific immunity. These immune modulating and immune evading properties are still unexplained.
Results
Here, we describe that ORFV infection of permissive cells impairs the intracellular transport of MHC class I molecules (MHC I) as a result of structural disruption and fragmentation of the Golgi apparatus. Depending on the duration of infection, we observed a pronounced co-localization of MHC I and COP-I vesicular structures as well as a reduction of MHC I surface expression of up to 50%. These subversion processes are associated with early ORFV gene expression and are accompanied by disturbed carbohydrate trimming of post-ER MHC I. The MHC I population remaining on the cell surface shows an extended half-life, an effect that might be partially controlled also by late ORFV genes.
Conclusions
The presented data demonstrate that ORFV down-regulates MHC I surface expression in infected cells by targeting the late vesicular export machinery and the structure and function of the Golgi apparatus, which might aid to escape cellular immune recognition.
【 授权许可】
2012 Rohde et al.; licensee BioMed Central Ltd.
【 预 览 】
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