Interspecies differences in membrane-associated protease activities of thyrocytes and their relevance for thyroid cancer studies

Journal of Experimental & Clinical Cancer Research

Richard Wahl²  Elke Maier³  Eleonore Fröhlich¹ 

[1] Center for Medical Research, Medical University of Graz, Stiftingtalstr 24, Graz, 8010, Austria;Department of Endocrinology, Metabolism, Nephrology and Clinical Chemistry, Internal Medicine, University of Tuebingen, Otfried-Muellerstrasse 10, 72076, Tuebingen, Germany;Institute of Anatomy, University of Tuebingen, Oesterbergstr 3, 72074, Tuebingen, Germany

关键词: Aminopeptidase N;    Dipeptidyl peptidase IV;    Protease activity;    Thyroid cancer;   

Others  :  826431
DOI  :  10.1186/1756-9966-31-45

 received in 2012-01-23, accepted in 2012-05-16,  发布年份 2012

【 摘 要 】

Background

To understand the role of proteases involved in human thyroid cancer progression and tissue invasion, thyrocytes from other species could potentially be used provided their characteristics are similar. It is not known whether dipeptidyl peptidase IV and aminopeptidase N activities, which are overexpressed in human thyroid cancer, are, as in human, also absent in normal thyrocytes of other species, making them suitable models for studies on the regulation of these proteases.

Methods

To assess the role of these proteases, activity was measured in thyroid tissue of human, mouse, rat, porcine, bovine and ovine origin. The lysosomal protease, dipeptidyl peptidase II, was used for comparison.

Results

Murine, rat, ovine, bovine and human thyrocytes all lacked dipeptidyl peptidase IV and aminopeptidase N activity, but porcine thyrocytes were found to possess both. In contrast, lysosomal dipeptidyl peptidase II was strongly expressed in all species. These activity patterns were maintained in cultured cells. Cultured porcine thyrocytes formed follicles with typical morphology upon stimulation with TSH but differed from human thyrocytes in their response to thiamazole.

Conclusions

These species differences in the expression of dipeptidyl peptidase IV and aminopeptidase N, indicate that porcine thyrocytes cannot be considered appropriate for the study of proteases in human cancer development.

【 授权许可】

   
2012 Fröhlich et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1 .

Figure 2 .

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