CD57+ T-cells are a subpopulation of T-follicular helper cells in nodular lymphocyte predominant Hodgkin lymphoma

Experimental Hematology & Oncology

Lydia Visser¹  Anke van den Berg¹  Bea Rutgers¹  Arjan Diepstra¹  Ahmad Sattarzadeh¹ 

[1] Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

关键词: TFH cells;    CD57+ T cells;    Nodular predominant Hodgkin lymphoma;   

Others  :  1227941
DOI  :  10.1186/s40164-015-0022-1

 received in 2015-07-17, accepted in 2015-09-03,  发布年份 2015

【 摘 要 】

Background

Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) is characterized by lymphocyte-predominant (LP) cells in a background of CD4+ CD57+ T-cells. These cells are normally present in the germinal center of lymphoid tissues. The cells rosetting LP cells are described to be PD-1 and BCL-6 positive, which are markers of T-follicular helper cells. This study was designed to address the question: are the CD57+ T cells in germinal centers of tonsil and NLPHL TFH cells?

Results

Immunohistochemistry was performed on tonsil and NLPHL. For tonsil, cells per germinal center and for NLPHL, the area around LP cells was counted. Cells rosetting LP cells were also determined. In addition, flowcytometrywas performed on cell suspensions. Cells directly rosetting LP cells are positive for CD57 and/or for two markers of T-follicular helper (TFH) cells, PD-1 and BCL-6. We show that in both tonsil and NLPHL more than 90 % of CD57+ T-cells are also positive for PD-1, whereas roughly half of the PD-1+ T-cells are CD57+. CD57+ cells co-express BCL-6 in tonsil and in the rosetting cells of NLPHL.

Conclusions

We conclude that CD57+ T-cells are TFH cells and form a subpopulation of TFH cells in tonsils and NLPHL.

【 授权许可】

   
2015 Sattarzadeh et al.

【 图 表 】

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