BMC Veterinary Research | |
The major acute phase proteins of bovine milk in a commercial dairy herd | |
Peter David Eckersall3  Hayley Haining1  Timothy Parkin1  Peter Hastie1  Mary Waterston2  Funmilola Clara Thomas3  | |
[1] School of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK;Institute of Infection Immunity and Inflammation, College of Veterinary Medical and Life Sciences, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK;Institute of Biodiversity, Animal Health and Comparative Medicine, College of Veterinary Medical and Life Sciences, University of Glasgow, Bearsden Rd, Glasgow G61 1QH, UK | |
关键词: Bovine mastitis; Somatic cell counts; C-reactive protein; Serum amyloid A; Haptoglobin; | |
Others : 1224345 DOI : 10.1186/s12917-015-0533-3 |
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received in 2015-03-13, accepted in 2015-08-06, 发布年份 2015 | |
【 摘 要 】
Background
Milk acute phase proteins (APP) have been identified and show promise as biomarkers of mastitis. However analysis of their profile in dairy cows from a production herd is necessary in order to confirm their benefits in mastitis diagnosis. The profiles of milk haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were determined in 54 composite milk (milk from all functional quarters of a cow’s udder collected in a common receptacle) samples (CMS) from a commercial dairy farm. Milk Hp was also determined in individual quarter milk (milk from a single udder quarter) samples (QMS) (n = 149) of the cows.
An ELISA was developed and validated for the determination of milk Hp while commercial kits were used for M-SAA3 and CRP assay respectively. Composite milk APP results were compared with cow factors including parity, stage of lactation, percentage protein and fat as well as somatic cell counts (SCC).
Results
Composite milk Hp ranged from <0.4–55 μg/ml with a median of 3.5 μg/ml; composite milk M-SAA3 ranged from <0.6–50 μg/ml and had a median of 1.2 μg/ml, while CRP ranged from <1.80–173 ng/ml and had a median of 24.6 ng/ml. Significant correlations were found between composite SCC and Hp (P-value <0.009) as well as parity and Hp (P < 0.009), but not between M-SAA3 and SCC, M-SAA3 and Hp, M-SAA3 and CRP or M-SAA3 and parity. Milk CRP was correlated with % fat (P = 0.002) and % protein (P = 0.001) of the milk samples. The lack of correlation of SCC with the M-SAA3 and CRP could result from these APP being more sensitive to intra-mammary infection than SCC. Quarter milk Hp had a range of <0.4–420 μg/ml with a median value of 3.6 μg/ml, with 92 % of samples below 20 μg/ml.
Conclusion
Baseline values of Hp, M-SAA3 and CRP were established in composite milk from cows with normal SCC on the dairy farm. Parity was recognized as a possible confounding factor when diagnosing mastitis using Hp. The value of the APP, Hp, M-SAA3 and CRP as substitutes or to complement SCC in indicating udder inflammation, was demonstrated.
【 授权许可】
2015 Thomas et al.
【 预 览 】
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【 参考文献 】
- [1]Hogeveen H, Huijps K, Lam T. Economic aspects of mastitis: New developments. N Z Vet J. 2011; 59:16-23.
- [2]Akerstedt M, Waller KP, Larsen LB, Forsback L, Sternesjo A. Relationship between haptoglobin and serum amyloid A in milk and milk quality. Int Dairy J. 2008; 18:669-674.
- [3]Akerstedt M, Waller KP, Sternesjo A. Haptoglobin and serum amyloid A in relation to the somatic cell count in quarter, cow composite and bulk tank milk samples. J Dairy Res. 2007; 74:198-203.
- [4]Dohoo IR, Meek AH. Somatic-Cell Counts in Bovine-Milk. Can Vet J. 1982; 23:119-125.
- [5]Pyorala S. Indicators of inflammation in the diagnosis of mastitis. Vet Res. 2003; 34:565-578.
- [6]Schalm O, Noorlander D. Experiments and observations leading to development of the California mastitis test. J Am Vet Med Assoc. 1957; 130:199-204.
- [7]Ceciliani F, Ceron JJ, Eckersall PD, Sauerwein H. Acute phase proteins in ruminants. J Proteomics. 2012; 75:4207-4231.
- [8]Chemonges S, Tung J-P, Fraser JF. Proteogenomics of selective susceptibility to endotoxin using circulating acute phase biomarkers and bioassay development in sheep: a review. Proteome Sci. 2014; 12(1):12. BioMed Central Full Text
- [9]Eckersall PD, Young FJ, McComb C, Hogarth CJ, Safi S, Weber A et al.. Acute phase proteins in serum and milk from dairy cows with clinical mastitis. Vet Rec. 2001; 148:35-41.
- [10]Lavery K, Way A, Killian G. Identification and immunohistochemical localization of a haptoglobin-like protein in the tissues and fluids of the bovine (Bos taurus) ovary and oviduct. Reproduction. 2003; 125:837-846.
- [11]Lecchi C, Dilda F, Sartorelli P, Ceciliani F. Widespread expression of SAA and Hp RNA in bovine tissues after evaluation of suitable reference genes. Vet Immunol Immunopathol. 2012; 145:556-562.
- [12]Schulze Willbrenning G, Hiss S, Theune C, Mielenz M, Schellander K, Sauerwein H. Gelatinase activities and haptoglobin concentrations in healthy and in degenerative articular cartilage of pigs. J Anim Physiol Anim Nutr. 2010; 94:757-766.
- [13]Lindahl M, Stahlbom B, Svartz J, Tagesson C. Protein patterns of human nasal and bronchoalveolar lavage fluids analyzed with two-dimensional gel electrophoresis. Electrophoresis. 1998; 19:3222-3229.
- [14]Nielsen BH, Jacobsen S, Andersen PH, Niewold TA, Heegaard PMH. Acute phase protein concentrations in serum and milk from healthy cows, cows with clinical mastitis and cows with extramammary inflammatory conditions. Vet Rec. 2004; 154:361-365.
- [15]Gronlund U, Sandgren CH, Waller KP. Haptoglobin and serum amyloid A in milk from dairy cows with chronic sub-clinical mastitis. Vet Res. 2005; 36:191-198.
- [16]Morimatsu M, Syuto B, Shimada N, Fujinaga T, Yamamoto S, Saito M et al.. Isolation and Characterization of Bovine Haptoglobin from Acute Phase Sera. J Biol Chem. 1991; 266:11833-11837.
- [17]Hiss S, Mielenz M, Bruckmaier RM, Sauerwein H. Haptoglobin concentrations in blood and milk after endotoxin challenge and quantification of mammary Hp mRNA expression. J Dairy Sci. 2004; 87:3778-3784.
- [18]Thielen MA, Mielenz M, Hiss S, Zerbe H, Petzl W, Schuberth HJ et al.. Short communication: Cellular localization of haptoglobin mRNA in the experimentally infected bovine mammary gland. J Dairy Sci. 2007; 90:1215-1219.
- [19]Lai IH, Tsao JH, Lu YP, Lee JW, Zhao X, Chien FL, et al. Neutrophils as one of the major haptoglobin sources in mastitis affected milk. Vet Res. 2009;40.
- [20]Thielen MA, Mielenz M, Hiss S, Sauerwein H. Qualitative detection of haptoglobin mRNA in bovine and human blood leukocytes and bovine milk somatic cells. Vet Med. 2005; 50:515-520.
- [21]Thielen MA, Mielenz M, Hiss S, Petzl W, Zerbe H, Schuberth J et al.. Regulation of haptoglobin (Hp) mRNA expression in the bovine mammary gland parenchyma during experimental mastitis. J Anim Sci. 2006; 84:336.
- [22]Morimatsu M, Sarikaputi M, Syuto B, Saito M, Yamamoto S, Naiki M. Bovine Haptoglobin - Single Radial Immunodiffusion Assay of Its Polymeric Forms and Dramatic Rise in Acute-Phase Sera. Vet Immunol Immunopathol. 1992; 33:365-372.
- [23]Eckersall PD, Duthie S, Safi S, Moffatt D, Horadagoda NU, Doyle S et al.. An automated biochemical assay for haptoglobin: Prevention of interference from albumin. Comp Haematol Int. 1999; 9:117-124.
- [24]McDonald TL, Larson MA, Mack DR, Weber A. Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum. Vet Immunol Immunopathol. 2001; 83:203-211.
- [25]Jacobsen S, Niewold TA, Kornalijnslijper E, Toussaint MJM, Gruys E. Kinetics of local and systemic isoforms of serum amyloid A in bovine mastitic milk. Vet Immunol Immunopathol. 2005; 104:21-31.
- [26]Eckersall PD, Young FJ, Nolan AM, Knight CH, McComb C, Waterston MM et al.. Acute phase proteins in bovine milk in an experimental model of Staphylococcus aureus subclinical mastitis. J Dairy Sci. 2006; 89:1488-1501.
- [27]Rossevatn K, Andresen PK, Sletten K, Husebekk A, Husby G, Nordstoga K et al.. The Complete Amino-Acid-Sequence of Bovine Serum Amyloid Protein-A (Saa) and of Subspecies of the Tissue-Deposited Amyloid Fibril Protein-A. Scand J Immunol. 1992; 35:217-224.
- [28]Black S, Kushner I, Samols D. C-reactive protein. J Biol Chem. 2004; 279:48487-48490.
- [29]Eckersall PD, Conner JG. Bovine and Canine Acute Phase Proteins. Vet Res Commun. 1988; 12:169-178.
- [30]Lee WC, Hsiao HC, Wu YL, Lin JH, Lee YP, Fung HP et al.. Serum C-reactive protein in dairy herds. Can J Vet Res. 2003; 67:102-107.
- [31]Schrodl W, Kruger M, Hien TT, Fuldner M, Kunze R. C-reactive protein as a new parameter of mastitis. Tierarztl Prax. 1995; 23(4):337-41.
- [32]Kruger M, Neumann A. Investigations to dynamics of C-reactive protein, somatic cell counts, lactose content, electrical conductivity in initial quarter milk samples of subclinically diseased quarters of the udders in relationship to bacteriological results. Tierarztl Prax Ausg G Grosstiere Nutztiere. 1999; 27:164-167.
- [33]Kalmus P, Simojoki H, Pyorala S, Taponen S, Holopainen J, Orro T. Milk haptoglobin, milk amyloid A, and N-acetyl-beta-D-glucosaminidase activity in bovines with naturally occurring clinical mastitis diagnosed with a quantitative PCR test. J Dairy Sci. 2013; 96:3662-3670.
- [34]Hirvonen J, Pyorala S, JousimiesSomer H. Acute phase response in heifers with experimentally induced mastitis. J Dairy Res. 1996; 63:351-360.
- [35]Larsen LB, Hinz K, Jorgensen ALW, Moller HS, Wellnitz O, Bruckmaier RM et al.. Proteomic and peptidomic study of proteolysis in quarter milk after infusion with lipoteichoic acid from Staphylococcus aureus. J Dairy Sci. 2010; 93:5613-5626.
- [36]Pedersen LH, Aalbaek B, Rontved CM, Ingvartsen KL, Sorensen NS, Heegaard PMH et al.. Early pathogenesis and inflammatory response in experimental bovine mastitis due to Streptococcus uberis. J Comp Pathol. 2003; 128:156-164.
- [37]Akerstedt M, Bjorck L, Waller KP, Sternesjo A. Biosensor assay for determination of haptoglobin in bovine milk. J Dairy Res. 2006; 73:299-305.
- [38]Tan X, Ding SQ, Hu YX, Li JJ, Zhou JY. Development of an immunosensor assay for detection of haptoglobin in mastitic milk. Vet Clin Pathol. 2012; 41:575-581.
- [39]Braceland M, McLoughlin MF, Tinsley J, Wallace C, Cockerill D, McLaughlin M et al. Serum enolase: a non-destructive biomarker of white skeletal myopathy during pancreas disease (PD) in Atlantic salmon Salmo salar L. J Fish Dis 2014, doi:. 10. 1111/jfd.12296 webcite
- [40]Pantoja JC, Hulland C, Ruegg PL. Dynamics of somatic cell counts and intramammary infections across the dry period. Prev Vet Med. 2009; 90:43-54.
- [41]Akerstedt M, Forsback L, Larsen T, Svennersten-Sjaunja K. Natural variation in biomarkers indicating mastitis in healthy cows. J Dairy Res. 2011; 78:88-96.
- [42]Berglund I, Pettersson G, Ostensson K, Svennersten-Sjaunja K. Quarter milking for improved detection of increased SCC. Reprod Domest Anim. 2007; 42:427-432.
- [43]Schwarz D, Diesterbeck US, Konig S, Brugemann K, Schlez K, Zschock M et al.. Microscopic differential cell counts in milk for the evaluation of inflammatory reactions in clinically healthy and subclinically infected bovine mammary glands. J Dairy Res. 2011; 78:448-455.
- [44]Madouasse A, Huxley J, Browne W, Bradley A, Green M. Somatic cell count dynamics in a large sample of dairy herds in England and Wales. Prev Vet Med. 2010; 96:56-64.
- [45]Anderson KL, Correa MT, Allen A, Rodriguez RR. Fresh cow mastitis monitoring on day 3 postpartum and its relationship to subsequent milk production. J Dairy Sci. 2010; 93:5673-5683.
- [46]Middleton JR, Hardin D, Steevens B, Randle R, Tyler JW. Use of somatic cell counts and California mastitis test results from individual quarter milk samples to detect subclinical intramammary infection in dairy cattle from a herd with a high bulk tank somatic cell count. J Am Vet Med Assoc. 2004; 224:419-423.
- [47]O’Reilly KM, Green MJ, Peeler EJ, Fitzpatrick JL, Green LE. Investigation of risk factors for clinical mastitis in British dairy herds with bulk milk somatic cell counts less than 150,000 cells/ml. Vet Rec. 2006; 158:649-653.
- [48]Safi S, Khoshvaghti A, Jafarzadeh SR, Bolourchi M, Nowrouzian I. Acute phase proteins in the diagnosis of bovine subclinical mastitis. Vet Clin Pathol. 2009; 38:471-476.
- [49]Molenaar AJ, Harris DP, Rajan GH, Pearson ML, Callaghan MR, Sommer L et al.. The acute-phase protein serum amyloid A3 is expressed in the bovine mammary gland and plays a role in host defence. Biomarkers. 2009; 14:26-37.