Kinetics of the porcine reproductive and respiratory syndrome virus (PRRSV) humoral immune response in swine serum and oral fluids collected from individual boars

BMC Veterinary Research

Jeffrey Zimmerman²  Chong Wang¹  Sergio Lizano⁴  Anna Rice⁴  Kent Schwartz²  Chris Olsen²  Yaowalak Panyasing²  Mark Engle³  Apisit Kittawornrat² 

[1] Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, IA, 50011, USA;Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA;PIC North America, 100 Bluegrass Commons Blvd, Hendersonville, TN, 37075, USA;IDEXX Laboratories, Inc, Westbrook, ME, 04092, USA

关键词: Antibody kinetics;    IgG;    IgA;    IgM;    ELISA;    Oral fluid;    PRRSV;   

Others  :  1119577
DOI  :  10.1186/1746-6148-9-61

 received in 2012-12-18, accepted in 2013-02-21,  发布年份 2013

【 摘 要 】

Background

The object of this study was to describe and contrast the kinetics of the humoral response in serum and oral fluid specimens during acute porcine reproductive and respiratory syndrome virus (PRRSV) infection. The study involved three trials of 24 boars each. Boars were intramuscularly inoculated with a commercial modified live virus (MLV) vaccine (Trial 1), a Type 1 PRRSV field isolated (Trial 2), or a Type 2 PRRSV field isolate (Trial 3). Oral fluid samples were collected from individual boars on day post inoculation (DPI) -7 and 0 to 21. Serum samples were collected from all boars on DPI −7, 0, 7, 14, 21 and from 4 randomly selected boars on DPI 3, 5, 10, and 17. Thereafter, serum and oral fluid were assayed for PRRSV antibody using antibody isotype-specific ELISAs (IgM, IgA, IgG) adapted to serum or oral fluid.

Results

Statistically significant differences in viral replication and antibody responses were observed among the three trials in both serum and oral fluid specimens. PRRSV serum IgM, IgA, and IgG were first detected in samples collected on DPI 7, 10, and 10, respectively. Oral fluid IgM, IgA, and IgG were detected in samples collected between DPI 3 to 10, 7 to 10, and 8 to 14, respectively.

Conclusions

This study enhanced our knowledge of the PRRSV humoral immune response and provided a broader foundation for the development and application of oral fluid antibody-based diagnostics.

【 授权许可】

   
2013 Kittawornrat et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Madar R, Straka S, Baska T: Detection of antibodies in saliva–an effective auxiliary method in surveillance of infectious diseases. Bratisl Lek Listy 2002, 103:38-41.
• [2]Corthier G: Swine fever: influence of passive immunity on pig immune response following vaccination with a live virus vaccine (Thiverval strain). Ann Rech Vet 1976, 7:361-372.
• [3]Corthier G, Aynaud J: Comparison of the immune response in serum and bucco-pharyngeal secretions following immunization by different routes with a live hog cholera virus vaccine (Thiverval strain). Ann Vet Res 1977, 8:159-165.
• [4]Prickett JR, Johnson J, Murtaugh MP, Puvanendiran S, Wang C, Zimmerman JJ, Opriessnig T: Prolonged Detection of PCV2 and Anti-PCV2 Antibody in Oral Fluids Following Experimental Inoculation. Transbound Emerg Dis 2011, 58:121-127.
• [5]Kittawornrat A, Prickett J, Wang C, Panyasing Y, Ballagi A, Rice A, Main R, Johnson J, Rademacher C, Hoogland M, Rowland R, Zimmerman J: Detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in oral fluid specimens using a commercial PRRSV serum antibody ELISA. J Vet Diagn Invest 2012, 24:262-269.
• [6]Prickett J, Simer R, Christopher-Hennings J, Yoon KJ, Evans RB, Zimmerman JJ: Detection of Porcine reproductive and respiratory syndrome virus infection in porcine oral fluid samples: a longitudinal study under experimental conditions. J Vet Diagn Invest 2008, 20:156-163.
• [7]Panyasing Y, Goodell CK, Wang C, Kittawornrat A, Prickett JR, Schwartz KJ, Ballagi A, Lizano S, Zimmerman JJ: Detection of Influenza A Virus Nucleoprotein Antibodies in Oral Fluid Specimens From Pigs Infected Under Experimental Conditions Using a Blocking ELISA. Transbound Emerg Disin press
• [8]De Buysscher E, Berman D: Secretory immune response in intestinal mucosa and salivary gland after experimental infection of pigs with transmissible gastroenteritis virus. Am J Vet Res 1980, 41:1214-1220.
• [9]Loftager M, Eriksen L, Nielsen R: Antibodies against Actinobacillus pleuropneumoniae serotype 2 in mucosal secretions and sera of infected pigs as demonstrated by an enzyme-linked immunosorbent assay. Res Vet Sci 1993, 54:57-62.
• [10]De Buysscher E, Dubois R: Detection of IgA anti-Escherichia coli plasma cells in the intestine and salivary glands of pigs orally and locally infected with E. coli. Adv Exp Med Biol 1978, 107:593-600.
• [11]Kittawornrat A, Prickett J, Chittick W, Wang C, Engle M, Johnson J, Patnayak D, Schwartz T, Whitney D, Olsen C, Schwartz K, Zimmerman J: Porcine reproductive and respiratory syndrome virus (PRRSV) in serum and oral fluid samples from individual boars: Will oral fluid replace serum for PRRSV surveillance? Virus Res 2010, 154:170-176.
• [12]Atkinson JC, Dawes C, Ericson T, Fox PC, Gandara BK, Malamud D, Mandel ID, Navazesh M, Tabak LA: Guidelines for saliva nomenclature and collection. Ann N Y Acad Sci 1993, 694:xi-xii.
• [13]Ebersole JL: Humoral immune responses in gingival crevice fluid: local and systemic implications. Periodontol 2003, 31:135-166.
• [14]Brandtzaeg PER: Do Salivary Antibodies Reliably Reflect Both Mucosal and Systemic Immunity? Ann N Y Acad Sci 2007, 1098:288-311.
• [15]Challacombe SJ, Russell MW, Hawkes J: Passage of intact IgG from plasma to the oral cavity via crevicular fluid. Clin Exp Immunol 1978, 34:417-422.
• [16]Johnson W, Roof M, Vaughn E, Christopher-Hennings J, Johnson CR, Murtaugh MP: Pathogenic and humoral immune responses to porcine reproductive and respiratory syndrome virus (PRRSV) are related to viral load in acute infection. Vet Immunol Immunopathol 2004, 102:233-247.
• [17]Martínez-Lobo FJ, Díez-Fuertes F, Segalés J, García-Artiga C, Simarro I, Castro JM, Prieto C: Comparative pathogenicity of type 1 and type 2 isolates of porcine reproductive and respiratory syndrome virus (PRRSV) in a young pig infection model. Vet Microbiol 2011, 154:58-68.
• [18]Joo HS, Park BK, Dee SA, Pijoan C: Indirect fluorescent IgM antibody response of pigs infected with porcine reproductive and respiratory syndrome virus. Vet Microbiol 1997, 55:303-307.
• [19]Loemba HD, Mounir S, Mardassi H, Archambault D, Dea S: Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus. Arch Virol 1996, 141:751-761.
• [20]Park BK, Joo HS, Dee SA, Pijoan C: Evaluation of an indirect fluorescent IgM antibody test for the detection of pigs with recent infection of porcine reproductive and respiratory syndrome virus. J Vet Diagn Invest 1995, 7:544-546.
• [21]Yoon KJ, Zimmerman JJ, Swenson SL, McGinley MJ, Eernisse KA, Brevik A, Rhinehart LL, Frey ML, Hill HT, Platt KB: Characterization of the humoral immune response to porcine reproductive and respiratory syndrome (PRRS) virus infection. J Vet Diagn Invest 1995, 7:305-312.
• [22]Prickett JR, Zimmerman JJ: The development of oral fluid-based diagnostics and applications in veterinary medicine. Anim Health Res Rev 2010, 11:207-216.
• [23]Kim HS, Kwang J, Yoon IJ, Joo HS, Frey ML: Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogenous subpopulation of MA-104 cells. Arch Virol 1993, 133:477-483.
• [24]Chittick WA, Stensland WR, Prickett JR, Strait EL, Harmon K, Yoon KJ, Wang C, Zimmerman JJ: Comparison of RNA extraction and real-time reverse transcription polymerase chain reaction methods for the detection of porcine reproductive and respiratory syndrome virus in porcine oral fluid specimens. J Vet Diagn Invest 2011, 23:248-253.