Murine esBAF chromatin remodeling complex subunits BAF250a and Brg1 are necessary to maintain and reprogram pluripotency-specific replication timing of select replication domains

Epigenetics & Chromatin

David M Gilbert³  Zhong Wang⁴  Gerald R Crabtree⁵  Jiong Tang⁵  Miguel A Esteban¹  Yong Fan²  Peng Fang²  Xiaolin Gao²  Dana Battaglia³  Vishnu Dileep³  Takayo Sasaki³  Tyrone Ryba³  Ienglam Lei⁴  Shin-ichiro Takebayashi³ 

[1] Stem Cell and Cancer Biology Group, Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China;Harvard Stem Cell Institute, 1350 Massachusetts Avenue, Cambridge, MA, 02138, USA;Department of Biological Science, Florida State University, 319 Stadium Drive, Tallahassee, FL, 32306, USA;Department of Cardiac Surgery, Cardiovascular Research Center, University of Michigan Medical School, North Campus Research Complex, Ann Arbor, MI, 48109, USA;Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA, 94305, USA

关键词: Developmental regulation;    Chromosome;    esBAF complex;    Replication timing;    Replication domains;   

Others  :  804947
DOI  :  10.1186/1756-8935-6-42

 received in 2013-09-09, accepted in 2013-12-02,  发布年份 2013

【 摘 要 】

Background

Cellular differentiation and reprogramming are accompanied by changes in replication timing and 3D organization of large-scale (400 to 800 Kb) chromosomal domains (‘replication domains’), but few gene products have been identified whose disruption affects these properties.

Results

Here we show that deletion of esBAF chromatin-remodeling complex components BAF250a and Brg1, but not BAF53a, disrupts replication timing at specific replication domains. Also, BAF250a-deficient fibroblasts reprogrammed to a pluripotency-like state failed to reprogram replication timing in many of these same domains. About half of the replication domains affected by Brg1 loss were also affected by BAF250a loss, but a much larger set of domains was affected by BAF250a loss. esBAF binding in the affected replication domains was dependent upon BAF250a but, most affected domains did not contain genes whose transcription was affected by loss of esBAF.

Conclusions

Loss of specific esBAF complex subunits alters replication timing of select replication domains in pluripotent cells.

【 授权许可】

   
2013 Takebayashi et al.; licensee BioMed Central Ltd.

【 图 表 】

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