The rheological properties of different GNPs

Lipids in Health and Disease

Mohamed Anwar K Abdelhalim¹ 

[1] Department of Physics and Astronomy, College of Science, King, Saud University, Saudi Arabia

关键词: temperature;    size;    rheological properties;    GNPs;   

Others  :  1160369
DOI  :  10.1186/1476-511X-11-14

 received in 2011-12-20, accepted in 2012-01-24,  发布年份 2012

【 摘 要 】

Background

Rheological analysis can be employed as a sensitive tool in predicting the physical properties of gold nanoparticles (GNPs). Understanding the rheological properties of GNPs can help to develop a better therapeutic cancer product, since these physical properties often link material formulation and processing stages with the ultimate end use. The rheological properties of GNPs have not been previously documented. The present study attempted to characterize the rheological properties of different sizes of GNPs at: 1) fixed temperature and wide range of shear rates; 2) varied temperature and fixed shear rate.

Methods

10, 20 and 50 nm GNPs was used in this study. Several rheological parameters of GNPs such as viscosity, torque%, shear stress and shear rate were evaluated using Brookfield LVDV-III Programmable rheometer supplied with temperature bath and controlled by a computer. To measure fluid properties (viscosity as function of shear rate), e.g., to determine whether the flow is Newtonian or non-Newtonian flow behaviour, and viscoelasticity (viscosity as function of temperature), rheological parameters were firstly measured at starting temperature of 37°C and wide range of shear rates from 375 to 1875 s^-1, and secondly at a gradual increase of temperature from 37 to 42°C and fixed shear rate of 1875 s^-1.

Results

The 10, 20 and 50 nm GNPs showed mean size of 9.45 ± 1.33 nm, 20.18 ± 1.80 nm, and 50 nm GNPs, respectively. The 10 and 20 nm GNPs showed spherical morphology while 50 nm GNPs showed hexagonal morphology using the transmission electron microscope (TEM). The relation between viscosity (cp) and shear rate (s^-1) for 10, 20 and 50 nm GNPs at a temperature of 37°C showed non-Newtonian behaviour. Although the relationship between SS (dyne/cm²) and SR (s^-1) for 10, 20 and 50 nm GNPs was linearly related however their fluid properties showed non-Newtonian behaviour.

Conclusions

The torque%, viscosity (cp) and SS (dyne/cm²) of all GNP sizes decreased with increasing the temperature and with decreasing the GNP size (for each fixed temperature value). For each shear rate value, the viscosity of all GNPs decreased with decreasing the GNP size. This study demonstrates that the physical, dimensional and morphological changes of GNPs have effective influence on their rheological properties. To understand and categorize the role of GNPs in drug delivery and cancer therapy, GNPs of varying size, number of particles, shape and surface should be taken into consideration. Moreover, further additional in vivo studies after administration of GNPs in rats should be performed to support this hypothesis.

【 授权许可】

   
2012 Abdelhalim; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Brust M, Bethell D, Kiely CJ, Schiffrin DJ: Self-assembled gold nanoparticle thin films with non-metallic optical and electronic properties. Langmuir 1998, 14:5425-5429.
• [2]Li H, Jiang L: A study on the sizes and concentrations of GNPs by spectra of absorption, resonance Rayleigh scattering and resonance non-linear scattering. Prog Chem 1997, 9:397-397.
• [3]Collier CP, Saykally RJ, Shiang JJ, Henrichs SE, Heath JR: Reversible tuning of silver quantum dot monolayers through the metal-insulator transition. Science 1997, 277:1978-1981.
• [4]Schmid G: Chemical synthesis of large metal clusters and their properties. Chem Rev 1992, 92:1709-1709.
• [5]Borm PJ, Kreyling WG: Toxicological hazards of inhaled nanoparticles - potential implications for drug delivery. J Nanoscience Nanotechnol 2004, 4:521-531.
• [6]MacNee W, Donaldson K: Mechanism of lung injury caused by PM10 and ultrafine particles with special reference to COPD. Eur Respir J 2003, 40:47S-51S.
• [7]BarathManiKanth S, Kalishwaralal K, Sriram M, Pandian SRK, Youn H, Eom S, Gurunathan G: Anti-oxidant effect of GNPs restrains hyperglycemic conditions in diabetic mice. Journal of Nanobiotechnology 2010, 8:16. BioMed Central Full Text
• [8]Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ: In vitro toxicity of nanoparticles in BRL- 3A rat liver cells. Toxicol in Vitro 2005, 19:975-983.
• [9]Schrand AM, Bradich-Stolle LK, Schlager JJ, Dai L, Hussain SM: Can silver nanoparticles be useful as potential biological labels? Nanotechnology 2008, 9:1-13.
• [10]Steffe J: Rheological Methods in Food Process Engineering. 2nd edition. Freeman Press, Mitchigan State University, USA; 1996:21.
• [11]Bishay IE: Rheological characterization of inulin. In Gums and Stabilizers for the Food Industry. Edited by Williams PA, Phillips GO. Royal Society of Chemistry, London, UK; 1998:403-408.
• [12]El-Nagar G, Clowes G, Tudoric CM, Kuri V, Brennan CS: Rheological quality and stability of yog ice cream with added inulin. International Journal of Dairy Technology 2002, 55:89-93.
• [13]Vardhanabhuti B, Foegeding EA: Rheological properties and characterization of polymerized whey protein isolates. Journal of Agricultural and Food Chemistry 1999, 47:3649-3655.
• [14]Zimeri JE, Kokini JL: Rheological properties of inulin-waxy maize starch systems. Carbohydrate Polymers 2003, 52:67-85.
• [15]Baskurt OK, Hardeman M, Rampling MW, Meiselman HJ: Handbook of Hemorheology and Hemodynamics. Amsterdam, Netherlands:IOS Press; 2007.
• [16]Baskurt OK, Meiselman HJ: Blood rheology and hemodynamics. Seminars in Thrombosis and Haemostasis 2003, 29(5):435-450.
• [17]Baskurt OK, Boynard M, Cokelet GC: New guidelines for hemorheological laboratory techniques. Clinical Hemorheology and Microcirculation 2009, 42:75-97.
• [18]Abdelhalim MAK: The effects of size and period of administration of GNPs on rheological parameters of blood plasma of rats over a wide range of shear rates: In vivo. Lipids in Health and Disease 2011, 10:191. BioMed Central Full Text
• [19]Reddy JK, Rao MS: Lipid metabolism and liver inflammation. II. Fatty liver disease and fatty acid oxidation. Am J Physiol Gastrointest Liver Physiol 2006, 290(5):G852-8.
• [20]Tedescoa S, Doyleb H, Blascoc J, Redmondb G, Sheehana D: Oxidative stress and toxicity of GNPs in Mytilus edulis. Aquatic Toxicology 2010, 100:178-186.
• [21]Senaratne RH, De Silva AD, Williams SJ, Mougous JD, Reader JR, Zhang TJ, Chan S, Sidders B, Lee DH, Chan J, Bertozzi CR, Riley LW: 5'-Adenosinephosphosulphate reductase (CysH) protects Mycobacterium tuberculosis against free radicals during chronic infection phase in mice. Mol Microbiol 2006, 59:1744-1753.
• [22]Pan Y, Leifert A, Ruau D, Neuss S, Bornemann J, Schmid G, Wolfgang B, Ulrich S, Willi J-D: GNPs of diameter 1.4 nm trigger necrosis by oxidative stress and mitochondrial damage. Small 2009, 5:2067-2076.
• [23]Lasagna-Reeves C, Gonzalez-Romero D, Barria MA, Olmedo I, Clos A, Sadagopa Ramanujam VM, Urayama A, Vergara L, Kogan MJ, Soto C: Bioaccumulation and toxicity of GNPs after repeated administration in mice. Biochemical and Biophysical Research Communications 2010, 393:649-655.
• [24]Nel A, Xia T, Mdler L, Li N: Toxic potential of materials at the nanolevel. Science 2006, 311:622-627.
• [25]Abdelhalim MAK, Jarrar BM: GNPs administration induced prominent inflammatory, central vein intima disruption, fatty change and Kupffer cells hyperplasia. Lipids in Health and Disease 2011, 10:133. BioMed Central Full Text