Intravital imaging in spontaneously hypertensive stroke-prone rats-a pilot study

Experimental & Translational Stroke Medicine

Stefanie Schreiber⁴  Christoph Kleinschnitz¹  Roxana O Carare³  Hans-Jochen Heinze⁵  Klaus Reymann⁵  Stine Mencl¹  Celine Z Bueche⁴  Cornelia Garz²  Stoyan Stoyanov⁴  Solveig Niklass² 

[1] Department of Neurology, University Hospital of Würzburg, Würzburg, Germany;Department of Neurology, Otto-von-Guericke-University, Leipziger Strasse 44, 39120 Magdeburg, Germany;Faculty of Medicine, University of Southampton, Southampton, UK;German Center for Neurodegenerative Diseases (DZNE), Magdeburg, Germany;Leibniz Institute for Neurobiology (LIN), Magdeburg, Germany

关键词: CSVD;    PM;    2 ;    Intravital imaging;    SHRSP;   

Others  :  861442
DOI  :  10.1186/2040-7378-6-1

 received in 2013-11-27, accepted in 2014-01-19,  发布年份 2014

【 摘 要 】

Background

There is growing evidence that endothelial failure and subsequent blood brain barrier (BBB) breakdown initiate cerebral small vessel disease (CSVD) pathology. In spontaneously hypertensive stroke-prone rats (SHRSP) endothelial damage is indicated by intraluminal accumulations of erythrocytes (erythrocyte thrombi) that are not observed with current magnetic resonance imaging techniques. Two-photon microscopy (2 PM) offers the potential for real-time direct detection of the small vasculature. Thus, within this pilot study we investigated the sensitivity of 2 PM to detect erythrocyte thrombi expressing initiating CSVD phenomena in vivo.

Methods

Eight SHRSP and 13 Wistar controls were used for in vivo imaging and subsequent histology with haematoxylin-eosin (HE). For 2 PM, cerebral blood vessels were labeled by fluorescent Dextran (70 kDa) applied intraorbitally. The correlation between vascular erythrocyte thrombi observed by 2 PM and HE-staining was assessed. Artificial surgical damage and parenchymal Dextran distribution were analyzed postmortem.

Results

Dextran was distributed within the small vessel walls and co-localized with IgG.

Artificial surgical damage was comparable between SHRSP and Wistar controls and mainly affected the small vasculature. In fewer than 20% of animals there was correlation between erythrocyte thrombi as observed with 2 PM and histologically with HE.

Conclusions

Contrary to our initial expectations, there was little agreement between intravital 2 PM imaging and histology for the detection of erythrocyte thrombi. Two-photon microscopy is a valuable technique that complements but does not replace the value of conventional histology.

【 授权许可】

   
2014 Niklass et al.; licensee BioMed Central Ltd.

【 图 表 】

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