Patterned polymer matrix promotes stemness and cell-cell interaction of adult stem cells

Journal of Biological Engineering

William H. Hofmeister¹  Hak-Joon Sung²  Alexander Terekhov¹  Spencer W. Crowder²  Daniel A. Balikov²  Lino Costa¹  Lucas H. Hofmeister² 

[1] Center for Laser Applications, University of Tennessee Space Institute, Tullahoma, TN, USA;Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA

关键词: Nanofiber;    Nanopore;    Polycaprolactone;    Matrix;    Stem cell;   

Others  :  1230534
DOI  :  10.1186/s13036-015-0016-x

 received in 2015-07-07, accepted in 2015-09-21,  发布年份 2015

【 摘 要 】

Background

The interaction of stem cells with their culture substrates is critical in controlling their fate and function. Declining stemness of adult-derived human mesenchymal stem cells (hMSCs) during in vitro expansion on tissue culture polystyrene (TCPS) severely limits their therapeutic efficacy prior to cell transplantation into damaged tissues. Thus, various formats of natural and synthetic materials have been manipulated in attempts to reproduce in vivo matrix environments in which hMSCs reside.

Results

We developed a series of patterned polymer matrices for cell culture by hot-pressing poly(ε-caprolactone) (PCL) films in femtosecond laser-ablated nanopore molds, forming nanofibers on flat PCL substrates. hMSCS cultured on these PCL fiber matrices significantly increased expression of critical self-renewal factors, Nanog and OCT4A, as well as markers of cell-cell interaction PECAM and ITGA2. The results suggest the patterned polymer fiber matrix is a promising model to maintain the stemness of adult hMSCs.

Conclusion

This approach meets the need for scalable, highly repeatable, and tuneable models that mimic extracellular matrix features that signal for maintenance of hMSC stemness.

【 授权许可】

   
2015 Hofmeister et al.

【 图 表 】

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