Significance of PRO2000/ANCCA expression, a novel proliferation-associated protein in hepatocellular carcinoma

Cancer Cell International

Linlang Guo²  Ying Guo¹  Zhenzhu Chen²  Luqiao Luo²  Jie Huang²  Jie Yang² 

[1] Department of Organ Transplantation, Zhujiang Hospital, Southern Medical University, Guangzhou, China;Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou, China

关键词: Immunohistochemistry;    Hepatocellular carcinoma (HCC);    Proliferation;    PRO2000/ANCCA;   

Others  :  792134
DOI  :  10.1186/1475-2867-14-33

 received in 2013-11-29, accepted in 2014-03-26,  发布年份 2014

【 摘 要 】

Background

PRO2000/ANCCA may be an important candidate gene which located within a region of chromosome 8q in hepatocellular carcinoma (HCC). However, its significance remains unclear. The aim of this study was to explore the clinical significance of PRO2000/ANCCA expression in HCC.

Methods

The correlations of PRO2000/ANCCA expression with clinicopathological factors and prognosis of HCC patients were analyzed. Expression of PRO2000/ANCCA, ki-67, cyclinD1, p53 and p21 was detected in HCCs from 107 patients along with corresponding non-tumor tissues by immunohistochemistry.

Results

PRO2000/ANCCA expression was present in 66 of 107 (64.94%) HCC specimens in which 36 of 76 (47.37%) in well differentiated tumors and 30 of 31 (96.77%) in poorly differentiated tumors respectively, while 8 (7.48%) in adjacent non-tumor tissues with scattered positive cells. PRO2000/ANCCA expression was associated with clinicopathological features such as histological differentiation, number of tumor nodules, TNM stage, tumor microsatellite, portal vein tumor thrombus and recurrence, but not with gender, age, tumor size, cirrhosis, HBV infection and serum fetoprotein (AFP) level. There was a close relationship between PRO2000/ANCCA and ki-67 and cyclinD1 in HCC. PRO2000/ANCCA immunopositivity was independent of p53 and p21^WAF1/Cip1.

Conclusions

Increased expression of PRO2000/ANCCA is associated with adverse outcome in patients with HCC and is a predictor of poor prognosis for HCC. PRO2000/ANCCA may be involved in the development of HCC and might promote cell proliferation through a p53/ P21^WAF1/Cip1-independent pathway.

【 授权许可】

   
2014 Yang et al.; licensee BioMed Central Ltd.

【 图 表 】

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