Use of ethanol extract of Mycobacterium bovis for detection of specific antibodies in sera of farmed red deer (Cervus elaphus) with bovine tuberculosis

BMC Veterinary Research

Shigetoshi Eda¹  John P Bannantine⁴  W Ray Waters⁴  J Frank T Griffin²  Colin G Mackintosh³  Rachel E Johnson¹  Ashutosh Wadhwa¹ 

[1] Department of Forestry, Wildlife and Fisheries, Center for Wildlife Health, University of Tennessee Institute of Agriculture, Knoxville, Tennessee 37996, USA;Disease Research Laboratory, University of Otago, Dunedin, New Zealand;AgResearch Invermay, P.O. Box 50034, Mosgiel, New Zealand;Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 50010, USA

关键词: Red deer;    Mycobacterium bovis;    ELISA;    Bovine tuberculosis;   

Others  :  1119382
DOI  :  10.1186/1746-6148-9-256

 received in 2013-08-08, accepted in 2013-12-06,  发布年份 2013

【 摘 要 】

Background

Bovine tuberculosis (bTB) in wildlife species poses a threat to domestic livestock in many situations. Control programs for bTB in livestock depend on testing and slaughtering the positive animals; however, the currently available diagnostic tests often have poor specificity. In our previous study, we developed a specific and sensitive enzyme linked immunosorbent assay (ELISA) for another mycobacterial disease – Johne’s disease, using surface antigens of Mycobacterium avium ssp. paratuberculosis (MAP) extracted by briefly agitating the bacilli in 80% ethanol solution. The ELISA test was named ethanol vortex ELISA (EVELISA). The objective of this study is to examine whether EVELISA technique could be used to specifically detect anti-Mycobacterium bovis (M. bovis) antibodies in the serum of M. bovis-infected farmed red deer (Cervus elaphus). We tested a total of 45 red deer serum samples, divided in 3 groups – uninfected animals (n = 15), experimentally infected with M. bovis (n = 15) and experimentally infected with MAP (n = 15).

Results

The presence of anti-M. bovis antibodies was tested using an ethanol extract of M. bovis. Without absorption of anti-MAP cross reactive antibodies, it was found that 13 out of the 15 MAP-infected animals showed high antibody binding. Using heat killed MAP as an absorbent of cross reactive antibodies, anti-M. bovis antibodies were detected in 86.7% of M. bovis-infected animals with minor false positive results caused by MAP infection.

Conclusions

The results from this study suggest that EVELISA may form a basis for a sensitive and specific test for the diagnosis of bTB in farmed red deer.

【 授权许可】

   
2013 Wadhwa et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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