Effect of Furin inhibitor on lung adenocarcinoma cell growth and metastasis

Cancer Cell International

Song-Tao Xu¹  Zhan-Yu Bei¹  Li Gao¹  Shu-Mei Rao¹  Wen-Juan Fan¹  Yong-Chao Ma¹ 

[1] Luo He Medical Colledge, Daxue Road, #148, Luohe City, Henan Province 462002, P.R. China

关键词: Migration;    Apoptosis;    Lung adenocarcinoma;    Fruin inhibitors;   

Others  :  791968
DOI  :  10.1186/1475-2867-14-43

 received in 2013-12-10, accepted in 2014-03-11,  发布年份 2014

【 摘 要 】

Background

To investigate the mechanisms of lung adenocarcinoma cell metastasis and provide a theoretical basis for the in-depth study of lung adenocarcinoma.

Methods

A549 cells are incubated with different concentrations of Furin inhibitor for indicated times. The proliferation and migration were confirmed with MTT, colony formation, wound Healing and Transwell assayes. Hochest 33342 / PI double staining was used to detect apoptosis. Cell migration and apoptosis associated proteins were analysed by enzyme-linked immunosorbent assay (ELISA) and western blot.

Results

We have found that Furin inhibitor play a significant role in inhibition A549 cell growth. And we also found cell migration was inhibited significantly upon Furin inhibitor treatment.

Conclusion

The proliferration and migration of A549 cell were inhibited by Furin inbitor through down-regulation the expression of migration and apoptosis related proteins.

【 授权许可】

   
2014 Ma et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Weir HK, Thun MJ, Hankey BF: Annual report to the nation on the status of cancer, 1975–2000, featuring the uses of surveillance data for cancer prevention and control. J Natl Cancer Inst 2003, 95(17):1276-1299.
• [2]Sakashita S, Sakashita M, Sound Tsao M: Genes and pathology of non-small cell lung carcinoma. Semin Oncol 2014, 41(1):28-39.
• [3]Thomas G: Furin at the cutting edge: from protein traffic to embryogenesis and disease. Nat Rev Mol Cell Biol 2002, 3(10):753-766.
• [4]Basak A, Chen A, Scamuffa N, Mohottalage D, Basak S, Khatib AM: Blockade of furin activity and furin-induced tumor cells malignant phenotypes by the chemically synthesized human furin prodomain. Curr Med Chem 2010, 17(21):2214-2221.
• [5]Couture F, D'Anjou F, Desjardins R, Boudreau F, Day R: Role of proprotein convertases in prostate cancer progression. Neoplasia 2012, 14(11):1032-1042.
• [6]Lalou C, Basak A, Mishra P, Mohanta BC, Banik R, Dinda B, Khatib AM: Inhibition of tumor cells proliferation and migration by the flavonoid furin inhibitor isolated from Oroxylum indicum. Curr Med Chem 2013, 20(4):583-591.
• [7]Cavallar U, Christofori G: Multitasking in tumor progression: signaling function of cell adhension molecules. Ann N YAcad Sci 2004, 10(14):58-66.
• [8]Bassi DE, Mahloogi H, Al-Saleem L: Elevated Furin expression in aggressive human head and neck tumors and tumor cell lines. Mol Carcinog 2001, 31(4):224-232.
• [9]Scamuffa N, Sfaxi F, Ma J, Lalou C, Seidah N, Calvo F, Khatib AM: Prodomain of the proprotein convertase subtilisin/kexin Furin (ppFurin) protects from tumor progression and metastasis. Carcinogenesis 2014, 35(3):528-536.
• [10]Mbikay M, Sirois F, Yao J: Comparative analysis of expression of the proprotein convertases furin, PACE4, PCI and PC2 inhuman lung tumours. Br J Cancer 1997, 75(10):1509-1514.
• [11]Gao J, Ding F, Liu Q, Yao Y: Knockdown of MACC1 expression suppressed hepatocellular carcinoma cell migration and invasion and inhibited expression of MMP2 and MMP9. Mol Cell Biochem 2012, 12:1545-1557.
• [12]Hotary KB, Allen ED, Brooks PC, Datta NS, Long MW, Weiss SJ: Membrane type I matrix metalloproteinase usurps tumor growth control imposed by the three-dimensional extracellular matrix. Cell 2003, 114(1):33-45.
• [13]Rozanov DV, Golubkov VS, Strongin AY: Membrane type-1 matrix metalloproteinase (MT1-MMP) protects malignant cells from tumoricidal activity of re-engineered anthrax lethal toxin. Int J Biochem Cell Biol 2005, 37(1):142-154.
• [14]Stacker SA, Caesar C, Baldwin ME: VEGF-D promotes the metastatic spread of tumor cells via the lymphatics. Nat Med 2001, 7:186-191.
• [15]Nagy JA, Vasile E, Feng D: Vascular permeability factor/vascular endothelial growth factor induces lymphangiogenesis as well as angiogenesis. J Exp Med 2002, 196:1497-1506.
• [16]Voura EB, English JL, Yu HY, Ho AT, Subarsky P, Hill RP, Hojilla CV, Khokha R: Proteolysis during tumor cell extravasation in vitro: metalloproteinase involvement across tumor cell types. PLoS One 2013, 8(10):e78413.