Variations in genome-wide RNAi screens: lessons from influenza research

Journal of Clinical Bioinformatics

Wen-Chi Su¹  King-Song Jeng⁴  Nai-Chi Hsu²  Yi-Chen Wu²  Michael MC Lai³  Yu-Chi Chou⁴ 

[1] China Medical University, Room 602, 6 F, Cancer Center Building, No. 6, Hsueh-Shih Road, Taichung 40402, Taiwan;National RNAi Core Facility Platform, Academia Sinica, Taipei 11529, Taiwan;Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 70101, Taiwan;Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan

关键词: Lentivirus;    Influenza virus;    Pooled shRNA screen;    Arrayed siRNA screen;    Genome-wide screen;    RNA interference (RNAi);   

Others  :  1145421
DOI  :  10.1186/s13336-015-0017-5

 received in 2014-12-05, accepted in 2015-02-19,  发布年份 2015

【 摘 要 】

Genome-wide RNA interference (RNAi) screening is an emerging and powerful technique for genetic screens, which can be divided into arrayed RNAi screen and pooled RNAi screen/selection based on different screening strategies. To date, several genome-wide RNAi screens have been successfully performed to identify host factors essential for influenza virus replication. However, the host factors identified by different research groups are not always consistent. Taking influenza virus screens as an example, we found that a number of screening parameters may directly or indirectly influence the primary hits identified by the screens. This review highlights the differences among the published genome-wide screening approaches and offers recommendations for performing a good pooled shRNA screen/selection.

【 授权许可】

   
2015 Chou et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

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