The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB

Cancer Cell International

Chien-Chih Chiu¹  Hui-Min David Wang⁴  Wen-Tsan Chang⁷  Chang-Yi Wu⁶  Hurng Wern Huang⁸  Wei-Chiao Chang²  Yao Fong³  Feng-Wei Chen¹  Jin-Ching Lee¹  Han-Lin Chou⁶  I-Ling Lin⁵ 

[1] Department of Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan;Department of Clinical Pharmacy; Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, School of Pharmacy, Taipei Medical University, Taipei, Taiwan;Chest Surgery, Chi-Mei Foundation Medical Center, Yung Kang City, Tainan, 901, Taiwan;Department of Fragrance and Cosmetic Science; Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan;Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan;Department of Biological Sciences, National Sun Yat-Sen University, 70 Lien Hai Road, Kaohsiung 804, Taiwan;Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Kaohsiung Medical University, Kaohsiung, Taiwan;Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan

关键词: cyclin A2;    survivin;    p-NFκB;    p-Akt;    Ceramides;    NSCLC;    Apoptosis;    Lung cancer;   

Others  :  792490
DOI  :  10.1186/1475-2867-14-1

 received in 2013-06-27, accepted in 2013-12-23,  发布年份 2014

【 摘 要 】

The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C₂-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C₂-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G₁ arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C₂-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.

【 授权许可】

   
2014 Lin et al.; licensee BioMed Central Ltd.

【 图 表 】

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