Pigs fed camelina meal increase hepatic gene expression of cytochrome 8b1, aldehyde dehydrogenase, and thiosulfate transferase

Journal of Animal Science and Biotechnology

William R Caine²  Tanya McDonald³  Pascale Duff¹  William Jon Meadus¹ 

[1] AAFC-Lacombe, 6000 C&E Trail, Lacombe, AB, Canada T4L 1 W1;Caine Research Consulting, Box #1124, Nisku, AB, Canada T9E 8A8;School of Innovation, Olds College, 4500-50th St., Olds, AB, Canada T4H 1R6

关键词: Pig liver;    Glucosinolates;    Gene expression;    Camelina meal;   

Others  :  821750
DOI  :  10.1186/2049-1891-5-1

 received in 2013-07-11, accepted in 2013-12-24,  发布年份 2014

【 摘 要 】

Camelina sativa is an oil seed crop which can be grown on marginal lands. Camelina seed oil is rich in omega-3 fatty acids (>35%) and γ-tocopherol but is also high in erucic acid and glucosinolates. Camelina meal, is the by-product after the oil has been extracted. Camelina meal was fed to 28 d old weaned pigs at 3.7% and 7.4% until age 56 d. The camelina meal supplements in the soy based diets, improved feed efficiency but also significantly increased the liver weights. Gene expression analyses of the livers, using intra-species microarrays, identified increased expression of phase 1 and phase 2 drug metabolism enzymes. The porcine versions of the enzymes were confirmed by real time PCR. Cytochrome 8b1 (CYP8B1), aldehyde dehydrogenase 2 (Aldh2), and thiosulfate transferase (TST) were all significantly stimulated. Collectively, these genes implicate the camelina glucosinolate metabolite, methyl-sulfinyldecyl isothiocyanate, as the main xeniobiotic, causing increased hepatic metabolism and increased liver weight.

【 授权许可】

   
2014 Meadus et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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