Nuclear export of VP19C is not essential for replication of herpes simplex virus type 1

Cell & Bioscience

Keshen Li²  Bin Zhao¹  Wandong Liang⁵  Haihong Zhou¹  Hua Tao¹  Lili Cui²  Guoda Ma²  Dongwei Mao³  You Li⁴ 

[1] Department of Neurology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China;Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital, of Guangdong Medical College, Zhanjiang 524001, China;Gynaecological and obstetrical department of the fourth affiliated hospital of Harbin Medical University, Harbin, Heilongjiang 150001, China;State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China;Renji College, Wenzhou Medical University, Wenzhou 325000, China

关键词: Replication;    Bacterial artificial chromosome (BAC);    Nuclear export signal (NES);    VP19C;   

Others  :  1149329
DOI  :  10.1186/2045-3701-4-55

 received in 2014-07-19, accepted in 2014-09-07,  发布年份 2014

【 摘 要 】

Background

Herpes simplex virus (HSV) type 1 has a 152 kb double-stranded DNA genome that may encode more than 80 gene products, many of which remain uncharacterized. The HSV-1 triplex is a complex of three protein subunits, VP19C and a dimer of VP23 that is essential for capsid assembly. Previous studies have demonstrated that HSV-1 VP19C contains an atypical nuclear localization signal and a functional nuclear export signal (NES), which are both important for the nucleocytoplasmic shuttling of VP19C. However, whether the VP19C NES is required for efficient HSV-1 production is unknown.

Findings

In the present study, a VP19C NES-mutated recombinant virus was generated by using bacterial artificial chromosome recombineering technology to investigate the role of VP19C nuclear export in HSV-1 replication. Our results demonstrate that the growth curves, plaque areas, subcellular localization and viral gene expression are indistinguishable between the VP19C NES-mutated virus and the wild-type virus.

Conclusions

Our findings reported herein indicate abrogation of the nuclear export of VP19C did not affect HSV-1 replication and viral gene expression.

【 授权许可】

   
2014 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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