期刊论文详细信息
Gut Pathogens
Characterization of the cytolethal distending toxin (typhoid toxin) in non-typhoidal Salmonella serovars
Martin Wiedmann1  Gerald E Duhamel2  Henk C den Bakker3  Barbara M Bowen1  Lorraine D Rodriguez-Rivera4 
[1] Department of Food Science, College of Agriculture and Life Sciences, Cornell University, 347 Stocking Hall, Ithaca 14853, NY, USA;Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca 14853, NY, USA;Department of Animal and Food Sciences, College of Agricultural Sciences and Natural Resources, Lubbock 79409, TX, USA;Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station 77843-4458, TX, USA
关键词: PltB;    PltA;    CdtB;    Typhoid toxin;    Toxin;    Typhi;    Non-typhoidal;    Salmonella;   
Others  :  1221725
DOI  :  10.1186/s13099-015-0065-1
 received in 2015-02-20, accepted in 2015-07-06,  发布年份 2015
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【 摘 要 】

Background

For many putative Salmonella enterica subsp. enterica virulence genes, functional characterization across serovars has been limited. Cytolethal distending toxin B (CdtB) is an incompletely characterized virulence factor that is found not only in Salmonella enterica subsp. enterica serovar Typhi (Salmonella Typhi) and dozens of Gram negative bacterial pathogens, but also in non-typhoidal Salmonella (NTS) serovars.

Methods

A comparative genomics approach was performed to characterize sequence conservation of the typhoid toxin (TT), encoded in the CdtB-islet, between Salmonella Typhi and NTS serovars. The cytotoxic activity of representative Salmonella enterica subsp. enterica serovars Javiana, Montevideo and Schwarzengrund strains and their respective isogenic cdtB mutants was determined in human intestinal epithelial Henle-407 cells by assessment of cell cycle progression of infected cells using fluorescence-activated cell sorting (FACS). Two-way analysis of variance (ANOVA) was used to determine whether cdtB deletion had a significant (p < 0.05) effect on the percentage of Henle-407 cells at each stage of the cell cycle.

Results

Here we show that a CdtB-islet encoding the cytolethal distending toxin B (CdtB), pertussis-like toxin A (PltA), and pertussis-like toxin B (PltB) is present in a dozen NTS serovars and that these proteins have a high level of sequence conservation and each form monophyletic clades with corresponding Salmonella Typhi genes. Human epithelial Henle-407 cells infected with three representative CdtB-encoding NTS serovars displayed G 2 /M phase cell cycle arrest that was absent in cells infected with corresponding isogenic cdtB null mutants (p < 0.0001 for the factor ∆cdtB deletion).

Conclusion

Our results show that CdtB encoded by NTS serovars has a genomic organization, amino acid sequence conservation and biological activity similar to the TT, and thus, may contribute to disease pathogenesis.

【 授权许可】

   
2015 Rodriguez-Rivera et al.

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