Anti-inflammatory effects of fish oil in ovaries of laying hens target prostaglandin pathways

Lipids in Health and Disease

Dale Buchanan Hales¹  Nawneet K Kurrey¹  Stacey R McGee¹  Carolynn C Small¹  Erfan Eilati¹ 

[1] Department of Physiology, Southern Illinois University Carbondale, School of Medicine, Life Science II, Room 250 (M/C 6512), 1125 Lincoln Drive, Carbondale, IL 62901, USA

关键词: Prostaglandin E₂;    Cyclooxygenase;    Inflammation;    Omega-3 fatty acids;    Laying hen;    Fish oil;   

Others  :  829502
DOI  :  10.1186/1476-511X-12-152

 received in 2013-09-03, accepted in 2013-10-18,  发布年份 2013

【 摘 要 】

Background

An effective way to control cancer is by prevention. Ovarian cancer is the most lethal gynecological malignancy. Progress in the treatment and prevention of ovarian cancer has been hampered due to the lack of an appropriate animal model and absence of effective chemo-prevention strategies. The domestic hens spontaneously develop ovarian adenocarcinomas that share similar histological appearance and symptoms such as ascites and metastasis with humans. There is a link between chronic inflammation and cancer. Prostaglandin E₂ (PGE₂) is the most pro-inflammatory ecoisanoid and one of the downstream products of two isoforms of cyclooxygenase (COX) enzymes: COX-1 and COX-2. PGE₂ exerts its effects on target cells by coupling to four subtypes of receptors which have been classified as EP1-4. Fish oil is a source of omega-3 fatty acids (OM-3FAs) which may be effective in prevention of ovarian cancer. Our objective was to assess the potential impact of fish oil on expression of COX enzymes, PGE₂ concentration, apoptosis and proliferation in ovaries of laying hens.

Methods

48 white Leghorn hens were fed 50, 100, 175, 375 and 700 mg/kg fish oil for 21 days. The OM3-FAs and omega-6 fatty acids contents of egg yolks were determined by Gas Chromatography. Proliferation, apoptosis, COX-1, COX-2 and prostaglandin receptor subtype 4 (EP4) protein and mRNA expression and PGE₂ concentration in ovaries were measured by PCNA, TUNEL, Western blot, quantitative real-time qPCR and ELISA, respectively.

Results

Consumption of fish oil increased the incorporation of OM-3FAs into yolks and decreased both COX-1 and COX-2 protein and mRNA expression. In correlation with COXs down-regulation, fish oil significantly reduced the concentrations of PGE₂ in ovaries. EP4 protein and mRNA expression in ovaries of hens was not affected by fish oil treatment. A lower dose of fish oil increased the egg laying frequency. 175 and 700 mg/kg fish oil reduced proliferation and 700 mg/kg increased apoptosis in hen ovaries.

Conclusions

Our findings suggest that the lower doses of fish oil reduce inflammatory PG and may be an effective approach in preventing ovarian carcinogenesis. These findings may provide the basis for clinical trials utilizing fish oil as a dietary intervention targeting prostaglandin biosynthesis for the prevention and treatment of ovarian cancer.

【 授权许可】

   
2013 Eilati et al.; licensee BioMed Central Ltd.

【 图 表 】

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