Implantation of silicon dioxide-based nanocrystalline hydroxyapatite and pure phase beta-tricalciumphosphate bone substitute granules in caprine muscle tissue does not induce new bone formation

Head & Face Medicine

C James Kirkpatrick²  Robert A Sader¹  Oliver Kuenzel²  Ines Willershausen³  Mike Barbeck²  Samuel E Udeabor¹  Shahram Ghanaati¹ 

[1] Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany;Institute of Pathology, REPAIR-Lab, Johannes Gutenberg University Mainz, Langenbeckstrasse 1, Mainz, 55101, Germany;Department for Operative Dentistry, Johannes Gutenberg University Mainz, Mainz, Germany

关键词: Ectopic bone formation;    Hydroxyapatite;    ß-tricalciumphosphate;    Nanocrystalline;    NanoBone;    Cerasorb;    Osteoinduction;   

Others  :  813796
DOI  :  10.1186/1746-160X-9-1

 received in 2012-10-14, accepted in 2012-12-10,  发布年份 2013

【 摘 要 】

Background

Osteoinductive bone substitutes are defined by their ability to induce new bone formation even at heterotopic implantation sites. The present study was designed to analyze the potential osteoinductivity of two different bone substitute materials in caprine muscle tissue.

Materials and methods

One gram each of either a porous beta-tricalcium phosphate (β-TCP) or an hydroxyapatite/silicon dioxide (HA/SiO₂)-based nanocrystalline bone substitute material was implanted in several muscle pouches of goats. The biomaterials were explanted at 29, 91 and 181 days after implantation. Conventional histology and special histochemical stains were performed to detect osteoblast precursor cells as well as mineralized and unmineralized bone matrix.

Results

Both materials underwent cellular degradation in which tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells and TRAP-negative multinucleated giant cells were involved. The ß-TCP was completely resorbed within the observation period, whereas some granules of the HA-groups were still detectable after 180 days. Neither osteoblasts, osteoblast precursor cells nor extracellular bone matrix were found within the implantation bed of any of the analyzed biomaterials at any of the observed time points.

Conclusions

This study showed that ß-TCP underwent a faster degradation than the HA-based material. The lack of osteoinductivity for both materials might be due to their granular shape, as osteoinductivity in goat muscle has been mainly attributed to cylindrical or disc-shaped bone substitute materials. This hypothesis however requires further investigation to systematically analyze various materials with comparable characteristics in the same experimental setting.

【 授权许可】

   
2013 Ghanaati et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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