Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis

Lipids in Health and Disease

Jalil Benyacoub³  Dirk Haller²  Serge Rezzi⁴  Viktoria Bastic Schmid³  Cecil Pace-Asciak¹  Sylviane Metairon⁴  Robert Mansourian³  Frederic Raymond⁴  Pia Lichti²  Francois-Pierre Martin⁴  Manuel Oliveira³  Viral Brahmbhatt³  Nabil Bosco³ 

[1] Research Institute, E. McMaster Building, The Hospital for Sick Children, Toronto, Canada;Technische Universität München, Biofunctionality, ZIEL–Research Center for Nutrition and Food Science, CDD - Center for Diet and Disease, Gregor-Mendel-Straße 2, Freising-Weihenstephan, 85350, Germany;Nestlé Research Center, Vers-chez-les-Blanc, Lausanne 26, CH-1000, Switzerland;Current address: Nestlé Institute of Health Sciences SA, EPFL campus, Quartier de l’innovation, Building G, Lausanne, 1015, Switzerland

关键词: Omega-3 fatty acids;    Docosahexaenoic acid;    Eicosapentaenoic acid;    Eicosanoids;    Inflammatory bowel disease;    Inflammation;   

Others  :  834605
DOI  :  10.1186/1476-511X-12-81

 received in 2013-04-12, accepted in 2013-05-24,  发布年份 2013

【 摘 要 】

Background

Inflammatory bowel diseases (IBD) are chronic intestinal inflammatory diseases affecting about 1% of western populations. New eating behaviors might contribute to the global emergence of IBD. Although the immunoregulatory effects of omega-3 fatty acids have been well characterized in vitro, their role in IBD is controversial.

Methods

The aim of this study was to assess the impact of increased fish oil intake on colonic gene expression, eicosanoid metabolism and development of colitis in a mouse model of IBD. Rag-2 deficient mice were fed fish oil (FO) enriched in omega-3 fatty acids i.e. EPA and DHA or control diet for 4 weeks before colitis induction by adoptive transfer of naïve T cells and maintained in the same diet for 4 additional weeks. Onset of colitis was monitored by colonoscopy and further confirmed by immunological examinations. Whole genome expression profiling was made and eicosanoids were measured by HPLC-MS/MS in colonic samples.

Results

A significant reduction of colonic proinflammatory eicosanoids in FO fed mice compared to control was observed. However, neither alteration of colonic gene expression signature nor reduction in IBD scores was observed under FO diet.

Conclusion

Thus, increased intake of dietary FO did not prevent experimental colitis.

【 授权许可】

   
2013 Bosco et al.; licensee BioMed Central Ltd.

【 图 表 】

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