Reproducibility and intraindividual variation over days in buccal cell DNA methylation of two asthma genes, interferon γ (IFNγ) and inducible nitric oxide synthase (iNOS)

Clinical Epigenetics

RL Miller³  FF Perera¹  MM Niedzwiecki¹  H Jiang²  K Moors²  JS Kuriakose²  DZ Torrone² 

[1] Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, 722 West 168th Street, New York, NY 10032, USA;Division of Pulmonary, Allergy and Critical Care of Medicine, PH8E, Columbia University Medical Center, 630 West 168th Street, New York, NY 10032, USA;Department of Pediatrics, Columbia University Medical Center, PH8E, 630 West 168th Street, New York, NY 10032, USA

关键词: inner city;    pediatric;    epigenetic regulation;    buccal mucosa;    iNOS;    IFNγ;    asthma;    methylation;   

Others  :  791468
DOI  :  10.1186/1868-7083-4-3

 received in 2011-07-19, accepted in 2012-02-01,  发布年份 2012

【 摘 要 】

The biological mechanisms responsible for the onset and exacerbation of asthma symptoms in children may involve the epigenetic regulation of inflammatory genes after environmental exposures. Using buccal cells, we hypothesized that DNA methylation in promoter regions of two asthma genes, inducible nitric oxide synthase (iNOS) and interferon γ (IFNγ), can vary over several days. Repeat buccal samples were collected 4 to 7 days apart from 34 children participating in the Columbia Center for Children's Environmental Health (CCCEH) birth cohort study. Several field duplicates (sequential collection of two samples in the field) and replicates (one sample pyrosequenced twice) also were collected to ensure consistency with collection and laboratory procedures. DNA methylation was assessed by pyrosequencing a PCR of bisulfite-treated DNA. We found that replicate and field duplicate samples were correlated strongly (r = 0.86 to 0.99, P < 0.05), while repeat samples demonstrated low within-subject correlations (r = 0.19 to 0.56, P = 0.06 to 0.30). Our data reveal DNA methylation as a dynamic epigenetic mechanism that can be accessed safely and reproducibly in an inner city pediatric cohort using non-invasive buccal swabs and pyrosequencing technology.

【 授权许可】

   
2012 Torrone et al; licensee BioMed Central Ltd.

【 图 表 】

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