Aquaporin-3 positively regulates matrix metalloproteinases via PI3K/AKT signal pathway in human gastric carcinoma SGC7901 cells

Journal of Experimental & Clinical Cancer Research

Zekuan Xu¹  Li Yang¹  Lizong Shen¹  Wenjie Zhang¹  Yong Xu¹  Hao Xu¹ 

[1] Department of General Surgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu Province, 210029, China

关键词: PI3K/AKT pathway;    Matrix metalloproteinase;    Aquaporin-3;   

Others  :  827291
DOI  :  10.1186/1756-9966-30-86

 received in 2011-07-20, accepted in 2011-09-25,  发布年份 2011

【 摘 要 】

Background

matrix metalloproteinases (MMPs) are produced by tumor cells, so they may be associated with tumor progression including invasion, migration, angiogenesis and metastasis. Aquaporin-3 (AQP3) also plays a critical role in gastric cancer cell migration and proliferation.

Methods

In this study, AQP3 was silenced or over-expressed in SGC7901 cells.

Results

We found a significant decrease in MT1-MMP, MMP-2, and MMP-9 expression after AQP3 knockdown, and a significant increase in MT1-MMP, MMP-2, and MMP-9 expression after AQP3 over-expression in SGC7901 cells. We also found that AQP3 silence led to a significant decrease of phosphorylation of ser⁴⁷³ in AKT in SGC7901 cells.

Conclusion

Our findings showed that AQP3 might positively regulate MMPs proteins expression through PI3K/AKT signal pathway in human gastric carcinoma SGC7901 cells.

【 授权许可】

   
2011 Xu et al; licensee BioMed Central Ltd.

【 图 表 】

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